Buffered bluing reagent, iCON™ Staining System

iCON™ Buffered Bluing Reagent is buffered to a pH of 8; this closely controlled pH balanced solution provides consistent and rapid bluing of hematoxylin-stained nuclei without the risk of losing tissue sections from slides. It also allows for a higher throughput of slides. High pH bluing reagents may detach tissue sections from slides, this becomes a critical factor with frozen sections when limited tissue sections are available.

This pH-balanced bluing reagent enhances nuclear chromatin detail and initiates an insoluble dye-lake complex with nuclear chromatin, resulting in a permanent crisp blue-purple stained nucleus. It is universal and may be used with progressive and regressive hematoxylin stains.

When using the iCON™ Bluing Reagent with a standard staining protocol, only thirty seconds to one minute exposure time is required and a one-minute water rinse after bluing. When used for frozen sections, a reduced time of five to ten seconds is sufficient with a water rinse of only twenty seconds. The time required for this reaction is significantly less than standard bluing reagents making it ideal for use in frozen sectioning when time is a critical factor.

Our buffered bluing reagent is a clear colorless solution that is stable for winter shipments in cold climates. It's not a concentrate and does not require diluting before use. Additionally, an antimicrobial agent is added allowing for a shelf life of two years.

Features:
- Buffered to a pH of 8
- Provides consistent and rapid bluing of hematoxylin-stained nuclei
- Enhances nuclear chromatin detail and initiates an insoluble dye-lake complex
- Universal and may be used with progressive and regressive hematoxylin stains
- Clear colorless solution
- Two year shelf life

Packaging options:
ES36551-4G: 4 x 1 Gallon
ES36551-1G: 1 Gallon

Product Documents:
Product Literature
Product Brochure
Product SDS