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E-Z 96® Fastfilter Plasmid Kit

SKU ES52381
$213.40 to $2,490.30
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Details

The E-Z 96® FastFilter Plasmid Kit is optimized for the parallel preparation of up to 96 plasmid minipreps in 1 hour. The Lysate Clearance Plate replaces the time-consuming centrifugation involved in clearing of bacterial alkaline lysates. This kit contains all of the components needed for plasmid DNA purification in a 96-well format. Purified plasmid DNA is suitable for the most sensitive downstream applications, such as routine screening or automated DNA sequencing. The E-Z 96® Fastfilter Plasmid Kits newly designed 96-well DNA plates are ideal for use with automated liquid handlers.

Features:
- Clear lysate with E-Z 96® Lysate Clearance Plate
- No resin, slurries, or alcohol precipitation
- High-purity plasmid DNA in less than 1 hour
- Reproducible yields of molecular biology grade plasmid DNA
- Fast, easy and convenient protocols

Specifications:
Prep count: 1,4 or 20 preps
Downstream application: Cloning; sanger sequencing etc.
Starting material: Per well: 1.0-1.5 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid type: High-copy, low-copy, cosmid DNA
Processing mode: 96-well, centrifugation or vacuum
Throughput: 96 samples per run
DNA binding technology: Silica 96-well plate
Lysate clearance method: Centrifugation or vacuum with lysate clearnce plate
Processing time: <1 hour
Yield: 15-25 µg for high copy-number; 0.1-5 µg for low copy-number
Special note: Lysate clearance plate for fast processing

FastFilter Plasmid Kit Figure

Figure 1. Plasmid DNA purified using the E-Z 96® FastFilter Plasmid Kit. DNA was isolated from 48 1 mL bacterial cultures frown overnight in LB culture using the E-Z 96® FastFilter Plasmid Kit and eluted in 150 µL elution buffer. Plasmid DNA (10% of total purified DNA) was analyzed on 1.0% agarose gel. Lanes 1-48 represent plasmid DNA from 48 different bacterial cultures.

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