Warthin-Starry Stain Kit
Details
Warthin-Starry Stain is a stain based in silver nitrate, also known as silver staining, and is commonly used in histology laboratories. Its main intention is for use in visualizing Spirochetes, but is also effective with Helicobacter pylori, Legionella pneumophila, and Cat Scratch Fever bacteria. Warthin-Starry stain works by binding the silver ions in the silver-nitrate reagent with certain protein groups, staining the important components of the sample black/brown over a soft yellow background. This stain kit may be used on formalin-fixed, paraffin-embedded, or frozen sections depending on your lab’s needs or intentions.
Helicobacter pylori: Black
Legionella pneumophila: Black
Spirochetes: Black
Cat Scratch Fever Bacteria: Black
Klebsiella: Brown/Black
Nuclei: Brown
Background: Yellow
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
- Has not been tested on and may not be suitable for frozen sections
Control Tissue: Any well fixed tissue
Preparation of Reducing Solution Prior to Beginning:
1. Warm Gelatin (4%), Acidulated to liquify prior to beginning. Prepare Reducing Solution in an unused plastic Slide Jar.
2. Combine:
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. For spirochete staining only, place slide in room temperature Spirochete Sensitizer for 5 minutes (Omit this step for other listed bacteria). Note: Use of Spirochete Sensitizer will not affect staining of Helicobacter pylori, Legionella pneumophila, or Cat Scratch Fever bacteria.
3. Pour 20ml of Silver Nitrate Solution (0.5%), Acidulated in an unused plastic staining jar and place in a waterbath at 65-70°C for 5 minutes.
4. Place slide in warmed Silver Nitrate Solution (0.5%), Acidulated and incubate for 3-5 minutes with repeated agitation.
5. Transfer slide into previously prepared Reducing Solution and agitate. Place staining jar in a waterbath at 65-70°C with frequent agitation until tissue section is brown (approximately 1-5 minutes).
6. Rinse slide carefully in hot tap water for 2 minutes.
7. Dehydrate slide through 3 changes of fresh Absolute Alcohol.
8. Clear and mount in synthetic resin.
Rapid Procedure (Microwave Oven):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. For spirochete staining only, place slide in room temperature Spirochete Sensitizer for 5 minutes (Omit this step for other listed bacteria). Note: Use of Spirochete Sensitizer will not affect staining of Helicobacter pylori, Legionella pneumophila, or Cat Scratch Fever bacteria.
3. Pour 20ml of Silver Nitrate Solution (0.5%), Acidulated in an unused plastic staining jar and microwave at full power for 30 seconds. Invert several times to equalize temperature.
4. Place slide in warmed Silver Nitrate Solution (0.5%), Acidulated and incubate for 5 minutes with repeated agitation.
5. Warm previously prepared Reducing Solution in microwave at full power for 20 seconds. Invert several times to equalize temperature.
6. Transfer slide into warmed Reducing Solution and with frequent agitation until tissue section is brown (approximately 2-5 minutes).
7. Rinse slide carefully in hot tap water for 2 minutes.
8. Dehydrate slide through 3 changes of fresh Absolute Alcohol.
9. Clear and mount in synthetic resin.
Click for Instructions for Use
Helicobacter pylori: Black
Legionella pneumophila: Black
Spirochetes: Black
Cat Scratch Fever Bacteria: Black
Klebsiella: Brown/Black
Nuclei: Brown
Background: Yellow
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
- Has not been tested on and may not be suitable for frozen sections
Control Tissue: Any well fixed tissue
Preparation of Reducing Solution Prior to Beginning:
1. Warm Gelatin (4%), Acidulated to liquify prior to beginning. Prepare Reducing Solution in an unused plastic Slide Jar.
2. Combine:
12.5 ml Gelatin (4%), Acidulated
20-30 Drops Silver Nitrate Solution (2%), Acidulated
7.5 ml Hydroquinone Solution (0.1%), Acidulated
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. For spirochete staining only, place slide in room temperature Spirochete Sensitizer for 5 minutes (Omit this step for other listed bacteria). Note: Use of Spirochete Sensitizer will not affect staining of Helicobacter pylori, Legionella pneumophila, or Cat Scratch Fever bacteria.
3. Pour 20ml of Silver Nitrate Solution (0.5%), Acidulated in an unused plastic staining jar and place in a waterbath at 65-70°C for 5 minutes.
4. Place slide in warmed Silver Nitrate Solution (0.5%), Acidulated and incubate for 3-5 minutes with repeated agitation.
5. Transfer slide into previously prepared Reducing Solution and agitate. Place staining jar in a waterbath at 65-70°C with frequent agitation until tissue section is brown (approximately 1-5 minutes).
6. Rinse slide carefully in hot tap water for 2 minutes.
7. Dehydrate slide through 3 changes of fresh Absolute Alcohol.
8. Clear and mount in synthetic resin.
Rapid Procedure (Microwave Oven):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. For spirochete staining only, place slide in room temperature Spirochete Sensitizer for 5 minutes (Omit this step for other listed bacteria). Note: Use of Spirochete Sensitizer will not affect staining of Helicobacter pylori, Legionella pneumophila, or Cat Scratch Fever bacteria.
3. Pour 20ml of Silver Nitrate Solution (0.5%), Acidulated in an unused plastic staining jar and microwave at full power for 30 seconds. Invert several times to equalize temperature.
4. Place slide in warmed Silver Nitrate Solution (0.5%), Acidulated and incubate for 5 minutes with repeated agitation.
5. Warm previously prepared Reducing Solution in microwave at full power for 20 seconds. Invert several times to equalize temperature.
6. Transfer slide into warmed Reducing Solution and with frequent agitation until tissue section is brown (approximately 2-5 minutes).
7. Rinse slide carefully in hot tap water for 2 minutes.
8. Dehydrate slide through 3 changes of fresh Absolute Alcohol.
9. Clear and mount in synthetic resin.
Click for Instructions for Use
| Components | Size | SDS Links |
|---|---|---|
| Spirochete Sensitizer | 125 mL | View SDS |
| Gelatin (4%), Acidulated | 2 x 65 mL | View SDS |
| Silver Nitrate Solution (0.5%), Acidulated | 125 mL | View SDS |
| Hydroquinone Solution (0.1%), Acidulated | 2 x 30 mL | View SDS |
| Silver Nitrate Solution (2%), Acidulated | 30 mL | View SDS |
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