Colloidal Iron Stain Kit
Details
The Colloidal Iron Stain Kit is intended for use in the histological visualization of acid mucopolysaccharides.
Acidic Sulfated Mucosubstances: Bright Blue
Collagen: Shades of Red
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue:
- Small Intestine
- Colon
Prepare the Following Solutions Immediately Before Use:
Working Colloidal Iron Solution:
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Place slide in Acetic Acid Solution (12%) for 30 seconds. Use once and discard.
3. Place slide in Working Colloidal Iron Solution for 30 minutes. Agitate solution several times during staining. Use once and discard.
4. Rinse thoroughly in 3 changes of Acetic Acid Solution (12%) for 2 minutes each. Use once and discard.
5. Stain slide in Working Iron Stain Solution for 10 minutes. Agitate solution several times during staining. Use once and discard.
6. Rinse in 3 changes of distilled water.
7. Stain slide in Van Gieson’s Solution for 30-45 seconds.
8. Dehydrate in 3 changes of absolute alcohol.
9. Clear, and mount in synthetic resin.
Click for Instructions for Use
Acidic Sulfated Mucosubstances: Bright Blue
Collagen: Shades of Red
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue:
- Small Intestine
- Colon
Prepare the Following Solutions Immediately Before Use:
Working Colloidal Iron Solution:
5 ml Acetic Acid Solution (12%)Working Iron Stain Solution:
15 ml Distilled Water
20 ml Colloidal Iron Stock Solution
20 ml Hydrochloric Acid Solution (1N)
20 ml Potassium Ferrocyanide Solution (3%)
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Place slide in Acetic Acid Solution (12%) for 30 seconds. Use once and discard.
3. Place slide in Working Colloidal Iron Solution for 30 minutes. Agitate solution several times during staining. Use once and discard.
4. Rinse thoroughly in 3 changes of Acetic Acid Solution (12%) for 2 minutes each. Use once and discard.
5. Stain slide in Working Iron Stain Solution for 10 minutes. Agitate solution several times during staining. Use once and discard.
6. Rinse in 3 changes of distilled water.
7. Stain slide in Van Gieson’s Solution for 30-45 seconds.
8. Dehydrate in 3 changes of absolute alcohol.
9. Clear, and mount in synthetic resin.
Click for Instructions for Use
Components | Size | SDS Links |
---|---|---|
Acetic Acid Solution (12%) | 500 mL | View SDS |
Hydrochloric Acid Solution (1N) | 125 mL | |
Potassium Ferrocyanide Solution (3%) | 125 mL | View SDS |
Colloidal Iron Stock Solution | 125 mL | View SDS |
Van Gieson’s Solution | 125 mL | View SDS |
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