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Colloidal Iron Stain Kit

SKU ES4804
$273.73
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Details

The Colloidal Iron Stain Kit is intended for use in the histological visualization of acid mucopolysaccharides.

Acidic Sulfated Mucosubstances: Bright Blue
Collagen: Shades of Red

Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents

Control Tissue:
- Small Intestine
- Colon

Prepare the Following Solutions Immediately Before Use:
Working Colloidal Iron Solution:
5 ml Acetic Acid Solution (12%)
15 ml Distilled Water
20 ml Colloidal Iron Stock Solution
Working Iron Stain Solution:
20 ml Hydrochloric Acid Solution (1N)
20 ml Potassium Ferrocyanide Solution (3%)

Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Place slide in Acetic Acid Solution (12%) for 30 seconds. Use once and discard.
3. Place slide in Working Colloidal Iron Solution for 30 minutes. Agitate solution several times during staining. Use once and discard.
4. Rinse thoroughly in 3 changes of Acetic Acid Solution (12%) for 2 minutes each. Use once and discard.
5. Stain slide in Working Iron Stain Solution for 10 minutes. Agitate solution several times during staining. Use once and discard.
6. Rinse in 3 changes of distilled water.
7. Stain slide in Van Gieson’s Solution for 30-45 seconds.
8. Dehydrate in 3 changes of absolute alcohol.
9. Clear, and mount in synthetic resin.

Click for Instructions for Use


ComponentsSizeSDS Links
Acetic Acid Solution (12%)500 mLView SDS
Hydrochloric Acid Solution (1N)125 mL 
Potassium Ferrocyanide Solution (3%)125 mLView SDS
Colloidal Iron Stock Solution125 mLView SDS
Van Gieson’s Solution125 mLView SDS

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