E-Z 96® Tissue DNA Kit
Details
By adapting Hi-Bind technology with 96-well plates, the E-Z 96® Tissue DNA Kit provides a high-throughput method to purify genomic DNA from whole blood, buccal swabs, mouse tail, rat tail, tissues and animal cells and tissues in a 96-well plate format. Purified DNA is suitable for most downstream applications such as PCR, restriction enzyme digestion, and hybridization techniques.
Features:
- Rapid, isolation of DNA from 96 samples in less than 1 hour
- Reliable, reproducible DNA purification from variety of samples
- Safe, no phenol/chloroform extractions
- Purified DNA suitable for most downstream application
Specifications:
Prep count: 1 or 4 preps
Starting amount: 20 mg
Starting material: Tissue, mouse tail, cultured cells, bacteria, blood
Elution volume: 150 µL
Technology: Silica 96-well plate
Processing mode: Manual
Throughput: 96
Genomic DNA purified from various chicken tissues using E-Z 96® Tissue DNA Kit is of good quality.
Figure 1. Genomic DNA purified using the E-Z 96® Tissue DNA Kit. Genomic DNA was extracted from chicken tissue: sample 1 from spleen, sample 2 from lung, sample 3 from muscle, and sample 4 from liver. The 5% of each eluate genomic DNA was analyzed on a 0.8% agarose gel to demonstrate the yield and quality of the DNA.
Genomic DNA purified from various chicken tissues using E-Z 96® Tissue DNA Kit performed successful PCR amplification.
Figure 2. Amplification of genomic DNA isolated from chicken tissue samples using the E-Z 96® Tissue DNA Kit. DNA was isolated from chicken tissue in duplicate: sample 1 from spleen, sample 2 from lung, sample 3 from muscle, and sample 4 from liver. A total of 2 µL of each eluate was amplified with a PCR mastermix and NS gene primers for a 750 bp product to determine whether inhibitors were present in the eluted DNA. Each lane contains 10% of PCR product separated on a 1% agarose gel.
Genomic DNA purified from various chicken tissues using E-Z 96® Tissue DNA Kit has excellent yield and quality.
Figure 3. Genomic DNA purified using the E-Z 96® Tissue DNA Kit. Genomic DNA was extracted from chicken tissue: sample 1 from spleen, sample 2 from lung, sample 3 from muscle, and sample 4 from liver. The 5% of each eluate genomic DNA was analyzed on a 0.8% agarose gel to demonstrate the yield and quality of the DNA.
Product Documents:
Product Flyer
Features:
- Rapid, isolation of DNA from 96 samples in less than 1 hour
- Reliable, reproducible DNA purification from variety of samples
- Safe, no phenol/chloroform extractions
- Purified DNA suitable for most downstream application
Specifications:
Prep count: 1 or 4 preps
Starting amount: 20 mg
Starting material: Tissue, mouse tail, cultured cells, bacteria, blood
Elution volume: 150 µL
Technology: Silica 96-well plate
Processing mode: Manual
Throughput: 96
Genomic DNA purified from various chicken tissues using E-Z 96® Tissue DNA Kit is of good quality.
Figure 1. Genomic DNA purified using the E-Z 96® Tissue DNA Kit. Genomic DNA was extracted from chicken tissue: sample 1 from spleen, sample 2 from lung, sample 3 from muscle, and sample 4 from liver. The 5% of each eluate genomic DNA was analyzed on a 0.8% agarose gel to demonstrate the yield and quality of the DNA.
Genomic DNA purified from various chicken tissues using E-Z 96® Tissue DNA Kit performed successful PCR amplification.
Figure 2. Amplification of genomic DNA isolated from chicken tissue samples using the E-Z 96® Tissue DNA Kit. DNA was isolated from chicken tissue in duplicate: sample 1 from spleen, sample 2 from lung, sample 3 from muscle, and sample 4 from liver. A total of 2 µL of each eluate was amplified with a PCR mastermix and NS gene primers for a 750 bp product to determine whether inhibitors were present in the eluted DNA. Each lane contains 10% of PCR product separated on a 1% agarose gel.
Genomic DNA purified from various chicken tissues using E-Z 96® Tissue DNA Kit has excellent yield and quality.
Figure 3. Genomic DNA purified using the E-Z 96® Tissue DNA Kit. Genomic DNA was extracted from chicken tissue: sample 1 from spleen, sample 2 from lung, sample 3 from muscle, and sample 4 from liver. The 5% of each eluate genomic DNA was analyzed on a 0.8% agarose gel to demonstrate the yield and quality of the DNA.
Product Documents:
Product Flyer
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