Elastic Stain Kit (Modified Verhoff's)
Details
The Elastic Stain Kit (Modified Verhoff's) is intended for use in the histological demonstration of elastin in tissue sections. Demonstration of elastic tissue is useful in cases of emphysema (atrophy of elastic tissue), arteriosclerosis (thinning and loss of elastic fibers) and various other vascular diseases.
Elastic Fibers: Black to Blue/Black
Nuclei: Blue to Black
Collagen: Red
Muscle & Other: Yellow
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue: Lung or any vascular tissue
Preparation of Reagents Prior to Beginning:
1. Prepare working Elastic Stain Solution by mixing 30ml Hematoxylin Solution (5%) with 12ml Ferric Chloride
Solution (10%) and 12ml Lugol’s Iodine Solution. Mixed solution may be used for 24 hours.
Note: Removal of mercury deposits is not required for tissues that have been fixed in mercury containing fixatives
since it will be removed by the staining solution.
Procedure:
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Place slides in working Elastic Stain Solution for 15 minutes.
3. Rinse in running tap water until no excess stain remains on slide.
4. Dip slides in Ferric Chloride (2%) Differentiating Solution 15-20 times and rinse in tap water.
5. Check slides microscopically for proper differentiation. Repeat step 4 if required.
6. Rinse in running tap water.
7. Place slides in Sodium Thiosulfate Solution (5%) for 1 minute.
8. Rinse in tap water.
9. Stain slide using Van Gieson’s Solution for 2-5 minutes.
10. Rinse in two changes of 95% alcohol.
11. Dehydrate in absolute alcohol.
12. Clear, and mount in synthetic resin.
Click for Instructions for Use
Elastic Fibers: Black to Blue/Black
Nuclei: Blue to Black
Collagen: Red
Muscle & Other: Yellow
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue: Lung or any vascular tissue
Preparation of Reagents Prior to Beginning:
1. Prepare working Elastic Stain Solution by mixing 30ml Hematoxylin Solution (5%) with 12ml Ferric Chloride
Solution (10%) and 12ml Lugol’s Iodine Solution. Mixed solution may be used for 24 hours.
Note: Removal of mercury deposits is not required for tissues that have been fixed in mercury containing fixatives
since it will be removed by the staining solution.
Procedure:
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Place slides in working Elastic Stain Solution for 15 minutes.
3. Rinse in running tap water until no excess stain remains on slide.
4. Dip slides in Ferric Chloride (2%) Differentiating Solution 15-20 times and rinse in tap water.
5. Check slides microscopically for proper differentiation. Repeat step 4 if required.
6. Rinse in running tap water.
7. Place slides in Sodium Thiosulfate Solution (5%) for 1 minute.
8. Rinse in tap water.
9. Stain slide using Van Gieson’s Solution for 2-5 minutes.
10. Rinse in two changes of 95% alcohol.
11. Dehydrate in absolute alcohol.
12. Clear, and mount in synthetic resin.
Click for Instructions for Use
| Components | Size | SDS Links |
|---|---|---|
| Hematoxylin Solution (5%) | 250 mL | View SDS |
| Ferric Chloride (10%, aqueous) | 125 mL | View SDS |
| Lugol’s Iodine Solution | 125 mL | View SDS |
| Ferric Chloride (2%) Differentiating Sol. | 125 mL | |
| Sodium Thiosulfate Solution | 125 mL | View SDS |
| Van Gieson’s Solution | 125 mL | View SDS |
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