H. Pylori Rapid Stain Kit
Details
The H. Pylori Rapid Stain Kit is intended for demonstrating Helicobacter Pylori infected tissue. Kit may be used on formalin fixed, paraffin-embedded tissue, or frozen sections.
Helicobacter Pylori: Blue
Mucin: Yellow
Background: Light Blue
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue: Helicobacter Pylori infected stomach
Prepare Toluidine Blue Working Solution (Immediately prior to use):
Distilled Water 50 ml
Toluidine Blue Solution 20 drops
Sodium Hydroxide Solution 3 drops
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Incubate slide in Periodic Acid Solution for 10 minutes (Note: Do Not Reuse Periodic Acid Solution).
3. Rinse thoroughly in distilled water.
4. Incubate slide in Sodium Metabisulfite Solution for 5 minutes.
5. Rinse thoroughly in distilled water.
6. Incubate slide in Alcian Yellow Solution for 5-10 minutes.
7. Rinse slide thoroughly in distilled water.
8. Incubate slide in freshly prepared Toluidine Blue Working Solution for 3-5 minutes (Note: Do Not Reuse Toluidine Blue Working Solution).
9. Rinse thoroughly in distilled water.
10. Dehydrate quickly in 2 changes of absolute alcohol.
11. Clear, and mount in synthetic resin.
Click for Instructions for Use
Helicobacter Pylori: Blue
Mucin: Yellow
Background: Light Blue
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue: Helicobacter Pylori infected stomach
Prepare Toluidine Blue Working Solution (Immediately prior to use):
Distilled Water 50 ml
Toluidine Blue Solution 20 drops
Sodium Hydroxide Solution 3 drops
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Incubate slide in Periodic Acid Solution for 10 minutes (Note: Do Not Reuse Periodic Acid Solution).
3. Rinse thoroughly in distilled water.
4. Incubate slide in Sodium Metabisulfite Solution for 5 minutes.
5. Rinse thoroughly in distilled water.
6. Incubate slide in Alcian Yellow Solution for 5-10 minutes.
7. Rinse slide thoroughly in distilled water.
8. Incubate slide in freshly prepared Toluidine Blue Working Solution for 3-5 minutes (Note: Do Not Reuse Toluidine Blue Working Solution).
9. Rinse thoroughly in distilled water.
10. Dehydrate quickly in 2 changes of absolute alcohol.
11. Clear, and mount in synthetic resin.
Click for Instructions for Use
| Components | Size | SDS Links |
|---|---|---|
| Periodic Acid Solution | 500 mL | View SDS |
| Sodium Metabisulfite Solution | 125 mL | View SDS |
| Alcian Yellow Solution | 125 mL | View SDS |
| Toluidine Blue Solution | 2 x 6 mL | View SDS |
| Sodium Hydroxide Solution | 8 mL | View SDS |
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