Harris Hematoxylin

Harris Hematoxylin is used for histology specimen staining; and this regressive stain appears darkest/most intense in appearance. Use this stain if the Gill 3X Hematoxylin staining is not providing a dark enough nuclear stain. Alternatively, use the Harris, Acidified as a progressive method for fewer steps and the same good quality.

Features:
- Used for histology specimen staining
- Use this stain if the Gill 3X Hematoxylin staining is not providing a dark enough nuclear stain

Specifications:
Size: 16oz or 1 Gallon bottle

Protocol:
1. Paraffin slides: Deparaffinize in xylene, then rehydrate through graded alcohol to water.
Frozen slides: Fix in Ethanol 95% (or other fixative according to lab preference) and rinse in water.
2. Stain in Hematoxylin solution, Harris Hematoxylin, acidified 2 to 4 minutes
3. Rinse in two changes of DI water: 1 minute each
Note: This could also be done using running tap water, but we recommend DI water to avoid the varying pH found in tap water.
4. Bluing Reagent (until tissue turns blue): 15 to 60 seconds
5. Rinse in two changes of DI water: 30-60 seconds
Note: This could also be done using running tap water.
6. Ethanol 95%: 15 to 30 seconds
7. Eosin Y 1% Alcoholic, Eosin Y Intensified, or Eosin-Phloxine: 30 to 60 seconds
Note: for an increased definition of cytoplasmic components over Eosin Y 1%, select Eosin Y Intensified or Eosin-Phloxine as a substitute.
8. Dehydrate in Ethanol 95%, two changes: 10 dips each
9. Dehydrate in Ethanol 100%, two changes: 1 minute each
10. Clear in Xylene or Xylene Substitute, two changes: 1 minute each.
11. Coverslip using Acrylic Mounting Medium and examine under microscope

Results:
Nuclei, nuclear components – blue to dark purple
Cytoplasm, red blood cells, collagen, elastic fiber, muscle –
shades of pink