Hepatitis B Stain Kit
Details
The Hepatitis B Stain Kit is intended for use in the histological visualization of Hepatitis B surface antigen (HBsAg). This kit may be used on formalin-fixed, paraffin-embedded or frozen sections.
HBsAg: Dark Brown/Purple
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue: Any well fixed section cut 3-5 microns
Prepare Oxidizing Immediately Prior to Beginning Procedure:
Combine:
50 ml Distilled Water
5 ml Potassium Permanganate Solution (5%)
3 ml Sulfuric Acid Solution (3%)
Mix thoroughly.
Procedure:
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Incubate slide in freshly prepared Oxidizing Solution for 10 minutes.
3. Rinse slide briefly in running tap water followed by 1 dip in distilled water.
4. Incubate slide in Oxalic Acid Solution (2%) for 10 minutes.
Note: Section should be colorless following this step.
5. Rinse slide for 1 minute in running tap water followed by 2 dips in distilled water.
6. Incubate slide in Orcein Solution for 2 hours.
7. Rinse slide in Reagent Alcohol (70%).
8. Apply Differentiating Solution for 3-5 seconds.
9. Dip slide in Reagent Alcohol (70%) and check slide microscopically for proper differentiation.
Note: Repeat step 8 if necessary.
10. Dehydrate quickly in 3 changes of absolute alcohol.
11. Clear, and mount in synthetic resin.
Click for Instructions for Use
HBsAg: Dark Brown/Purple
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue: Any well fixed section cut 3-5 microns
Prepare Oxidizing Immediately Prior to Beginning Procedure:
Combine:
50 ml Distilled Water
5 ml Potassium Permanganate Solution (5%)
3 ml Sulfuric Acid Solution (3%)
Mix thoroughly.
Procedure:
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Incubate slide in freshly prepared Oxidizing Solution for 10 minutes.
3. Rinse slide briefly in running tap water followed by 1 dip in distilled water.
4. Incubate slide in Oxalic Acid Solution (2%) for 10 minutes.
Note: Section should be colorless following this step.
5. Rinse slide for 1 minute in running tap water followed by 2 dips in distilled water.
6. Incubate slide in Orcein Solution for 2 hours.
7. Rinse slide in Reagent Alcohol (70%).
8. Apply Differentiating Solution for 3-5 seconds.
9. Dip slide in Reagent Alcohol (70%) and check slide microscopically for proper differentiation.
Note: Repeat step 8 if necessary.
10. Dehydrate quickly in 3 changes of absolute alcohol.
11. Clear, and mount in synthetic resin.
Click for Instructions for Use
| Components | Size | SDS Links |
|---|---|---|
| Potassium Permanganate Solution (5%) | 30 mL | View SDS |
| Sulfuric Acid Solution (3%) | 30 mL | View SDS |
| Oxalic Acid Solution (2%) | 125 mL | View SDS |
| Orcein Solution | 125 mL | View SDS |
| Differentiating Solution | 125 mL | View SDS |
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