Mucicarmine Stain Kit (Modified Southgate's)
Details
Mucicarmine Stain Kit (Modified Southgate's) is intended for use in the histological visualization of acid mucopolysacharides in tissue sections. This product is useful in distinguishing mucin negative undifferentiated squamous cell lesions from mucin positive adenocarcinomas. In addition, this product will stain the mucopolysaccharide capsule of Cryptococcus neoformans.
What is Mucicarmine Stain?
There are a variety of mucin stains, each similar while also different in their attempt distinguish several types of mucopolysaccharide substances in gastrointestinal tissue samples.
Mucin: Pink/Red
Capsule of Cryptococcus: Red
Nuclei: Black/Blue
Other Tissue Components: Yellow
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue:
- Colon
- Intestine
- Bronchi Epithelial cells
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. If sections are Zenker-fixed, remove mercuric chloride crystals using iodine and clear with sodium thiosulfate. Rinse in running tap water.
3. Stain slide in Hematoxylin for 2-3 minutes.
4. Rinse for 2 minutes in running tap water.
5. Dip slide in Bluing Reagent for 30 seconds.
6. Rinse slide in distilled water.
7. Stain slide in Mucicarmine Solution for 10 minutes.
8. Rinse quickly in tap water followed by a quick rinse in distilled water.
9. Stain slide in Tartrazine Solution for 1 minute.
10. Rinse quickly in tap water followed by a quick rinse in distilled water.
11. Dehydrate through graded alcohols.
12. Clear, and mount in synthetic resin.
Click for Instructions for Use
What is Mucicarmine Stain?
There are a variety of mucin stains, each similar while also different in their attempt distinguish several types of mucopolysaccharide substances in gastrointestinal tissue samples.
Mucin: Pink/Red
Capsule of Cryptococcus: Red
Nuclei: Black/Blue
Other Tissue Components: Yellow
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue:
- Colon
- Intestine
- Bronchi Epithelial cells
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. If sections are Zenker-fixed, remove mercuric chloride crystals using iodine and clear with sodium thiosulfate. Rinse in running tap water.
3. Stain slide in Hematoxylin for 2-3 minutes.
4. Rinse for 2 minutes in running tap water.
5. Dip slide in Bluing Reagent for 30 seconds.
6. Rinse slide in distilled water.
7. Stain slide in Mucicarmine Solution for 10 minutes.
8. Rinse quickly in tap water followed by a quick rinse in distilled water.
9. Stain slide in Tartrazine Solution for 1 minute.
10. Rinse quickly in tap water followed by a quick rinse in distilled water.
11. Dehydrate through graded alcohols.
12. Clear, and mount in synthetic resin.
Click for Instructions for Use
Components | Size | SDS Links |
---|---|---|
Hematoxylin, Mayer’s | 125 mL | View SDS |
Bluing Reagent | 125 mL | View SDS |
Mucicarmine Solution | 125 mL | View SDS |
Tartrazine Solution | 125 mL | View SDS |
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