CD163
Details
CD163 is a protein encoded by the CD163 gene in humans. It serves as a high-affinity scavenger receptor for the hemoglobin-haptoglobin complex. In the absence of haptoglobin, it can also bind to hemoglobin alone, albeit with lower affinity. CD163 is exclusively expressed on the cell surface of human monocytes and macrophages, particularly those that are involved in the late phase of inflammation. Therefore, it is a valuable marker for macrophage phenotyping.CD163 is a protein encoded by the CD163 gene in humans. It serves as a high-affinity scavenger receptor for the hemoglobin-haptoglobin complex. In the absence of haptoglobin, it can also bind to hemoglobin alone, albeit with lower affinity. CD163 is exclusively expressed on the cell surface of human monocytes and macrophages, particularly those that are involved in the late phase of inflammation. Therefore, it is a valuable marker for macrophage phenotyping.
Immunohistochemistry (IHC) using CD163 antibodies can detect CD163 positivity in histiocytes, gut cells, Kupffer cells, select alveolar macrophages, the main population of macrophages in the placenta, and varying degrees of macrophages in inflamed tissue, including tumor tissue, depending on the stage of inflammation. CD163 positivity is observed in red-pulp macrophages (but not white-pulp macrophages) in the spleen and cortical macrophages in the thymus. In the context of rheumatoid arthritis, CD163 antibody has been found to be helpful in differentiating synovial macrophages from synovial intimal fibroblasts, demonstrating superior specificity for macrophages compared to CD68, which does not discriminate between these cell types. CD163 antibody has also been implicated in the prognostic evaluation of tumor-infiltrating macrophages in classical Hodgkin lymphoma. Increased levels of CD163 have been detected in patients with microbial infections and myelomonocytic leukemias, and CD163 antibody expression has been shown to be limited to leukemias with monocytic differentiation. Recent studies have reported positive CD163 expression in all five cases of synovial-type giant cell tumors of the spinal column.
| Specifications: | |
|---|---|
| Intended Use: | This antibody is intended for use in Immunohistochemical applications on formalin-fixed paraffin-embedded tissues (FFPE). The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the of the patient's clinical history and diagnostic tests by a qualified pathologist. |
| Format: | Prediluted, Concentrated |
| Antibody type: | Mouse Monoclonal |
| Isotype: | IgG |
| Localization: | Membranous |
| Clone: | C2F5 |
| Reactivity: | Human Paraffin |
| Preparation: | This antibody is derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide. |
Prediluted:
1mL: ES35811-P-1ML
3mL: ES35811-P-3ML
6mL: ES35811-P-6ML
Concentrated:
0.1mL: ES35811-C-01ML
0.2mL: ES35811-C-05ML
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