Combined Eosinophil-Mast Cell Stain Kit
Details
The Combined Eosinophil-Mast Cell Stain Kit is intended for simultaneous visualization of eosinophils and mast cells.
Mast Cells: Bright Blue
Eosinophils: Bright Red
Nuclei: Blue
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue: Any well fixed section cut 3-5 microns or any air dried smear on clean slide
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Rinse for 2 minutes in running tap water followed by 2 changes of distilled water.
3. Incubate slide in Astra Blue Solution for 1-3 minutes with frequent agitation.
4. Rinse quickly in tap water followed by a quick rinse in distilled water.
5. Incubate slide in Vital New Red Solution for 30 minutes with frequent agitation.
6. Rinse quickly in tap water followed by a quick rinse in distilled water.
7. Counterstain in Hematoxylin, Mayer’s (Lillie’s Modification) for 15-30 seconds.
8. Rinse slide in running tap water for 2 minutes.
9. Dehydrate quickly through 3 changes in absolute alcohol.
10. Clear, and mount in synthetic resin.
Click for Instructions for Use
Mast Cells: Bright Blue
Eosinophils: Bright Red
Nuclei: Blue
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue: Any well fixed section cut 3-5 microns or any air dried smear on clean slide
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Rinse for 2 minutes in running tap water followed by 2 changes of distilled water.
3. Incubate slide in Astra Blue Solution for 1-3 minutes with frequent agitation.
4. Rinse quickly in tap water followed by a quick rinse in distilled water.
5. Incubate slide in Vital New Red Solution for 30 minutes with frequent agitation.
6. Rinse quickly in tap water followed by a quick rinse in distilled water.
7. Counterstain in Hematoxylin, Mayer’s (Lillie’s Modification) for 15-30 seconds.
8. Rinse slide in running tap water for 2 minutes.
9. Dehydrate quickly through 3 changes in absolute alcohol.
10. Clear, and mount in synthetic resin.
Click for Instructions for Use
| Components | Size | SDS Links |
|---|---|---|
| Astra Blue Solution | 125 mL | View SDS |
| Vital New Red Solution | 125 mL | View SDS |
| Hematoxylin, Mayer’s | 125 mL | View SDS |
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