E.Z.N.A.® FastFilter Plasmid DNA Mini Kit
Details
The E.Z.N.A.® FastFilter Plasmid DNA Mini Kit is designed for rapid purification of high-quality plasmid DNA starting with bacterial pellet from up to 5 mL of culture following the alkaline-lysis method in just 9 minutes. The kit features an innovative and first of its kind FastFilter mini column nested within a regular HiBind® silica mini spin column to combine lysate clearance and DNA binding into one simple centrifugation step. Following wash and elute steps, purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.
Features:
- Rapid, 9 Minute Processing time from a bacterial pellet
- Unique Lysate Clearance and DNA Binding in 1 step
- Convenient Lysate Clearance Column eliminates cellular debris pelleting and transfer step
- Versatile, Process up to 5 mL bacterial cultures with different plasmid types and culture medias
Specifications:
Prep count: 10, 100, or 300 preps
Starting amount: up to 5mL (high-copy number plasmid) and 10mL (low-copy number plasmid)
Starting material: Bacteria harboring high-copy or low-copy plasmid in culture medium
Elution volume: 50 - 100μL
Processing mode: Manual (Centrifugation or Vacuum)
Throughput: 1-24 Samples
Nucleic acid binding technology: Silica Mini Spin Column
Processing time: 9 minutes
Downstream application(s): Routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing
Special Features: First of its kind FastFilter and HiBind® DNA mini column configuration integrating lysate clearance and DNA binding into a single step
FASTER PROCESSING WITHOUT SACRIFICING YIELD AND QUALITY
Table 1. Competitor Analysis of Plasmid DNA Purification. pGEM plasmid was purified DH5α cultures (n = 4) using kits from Omega Bio-tek or Company Z or Company Q following manufacturer’s recommended centrifugation protocol. Purified Plasmid DNA was eluted at 100μL irrespective of the manufacturing kit used and quantified using Thermo Scientific’s NanoDrop™ 2000c system.
COMPARISON OF PROCESSING TIME FROM BACTERIAL CULTURE
Figure 1. Comparison of Plasmid Purification times (n = 4) using kits from Omega Bio-tek or Company Z or Company Q. The processing time was comparable using kits from Omega Bio-tek and Company Z at minimum culture volume and was lower by 2 min for Omega Bio-tek at maximum volume. The processing times shown are actual timed extractions including all pipetting, centrifugation, and bacterial culture pelleting steps. Omega Bio-tek outperformed Company Q with respect to processing time at both the culture volumes tested.
HIGH-QUALITY PLASMID DNA SUITABLE FOR SEQUENCING REACTIONS
Table 2. Plasmid DNA purified using the E.Z.N.A. FastFilter Plasmid DNA Mini Kit was used in a 5 μL Sanger sequencing reaction. The sequencing reaction was analyzed on an Applied Biosystems 3730XL. The samples had an average CRL of 994 bp and an average QV20+ of 985 with a quality score greater than 20 (≤ 1% probability of error in base calling).
VERSATILITY USING DIFFERENT PLASMID TYPES AND CULTURE MEDIA
Figure 2. E.Z.N.A.® FastFilter Plasmid DNA Mini Kit is capable of handling diverse sample input conditions ranging from plasmid types, culture medium as well as input culture volume. The plasmid yield may vary based on these conditions.
COMPLETE DIGESTION WITH VARIOUS RESTRICTION ENZYMES
Figure 3. pGEM Plasmid DNA from overnight bacterial cultures was purified using the E.Z.N.A. FastFilter Plasmid DNA Mini Kit and analyzed by restriction digestion. Complete digestion was observed for the various restriction enzymes tested.
HIGH-QUALITY, SUPERCOILED PLASMID DNA
Figure 4. Plasmid DNA Quality Assessment using kits from Omega Bio-tek or Company Q or Company Z. No genomic DNA was present on the agarose gel.
Product Documents:
Product Flyer
Features:
- Rapid, 9 Minute Processing time from a bacterial pellet
- Unique Lysate Clearance and DNA Binding in 1 step
- Convenient Lysate Clearance Column eliminates cellular debris pelleting and transfer step
- Versatile, Process up to 5 mL bacterial cultures with different plasmid types and culture medias
Specifications:
Prep count: 10, 100, or 300 preps
Starting amount: up to 5mL (high-copy number plasmid) and 10mL (low-copy number plasmid)
Starting material: Bacteria harboring high-copy or low-copy plasmid in culture medium
Elution volume: 50 - 100μL
Processing mode: Manual (Centrifugation or Vacuum)
Throughput: 1-24 Samples
Nucleic acid binding technology: Silica Mini Spin Column
Processing time: 9 minutes
Downstream application(s): Routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing
Special Features: First of its kind FastFilter and HiBind® DNA mini column configuration integrating lysate clearance and DNA binding into a single step
FASTER PROCESSING WITHOUT SACRIFICING YIELD AND QUALITY
Table 1. Competitor Analysis of Plasmid DNA Purification. pGEM plasmid was purified DH5α cultures (n = 4) using kits from Omega Bio-tek or Company Z or Company Q following manufacturer’s recommended centrifugation protocol. Purified Plasmid DNA was eluted at 100μL irrespective of the manufacturing kit used and quantified using Thermo Scientific’s NanoDrop™ 2000c system.
COMPARISON OF PROCESSING TIME FROM BACTERIAL CULTURE
Figure 1. Comparison of Plasmid Purification times (n = 4) using kits from Omega Bio-tek or Company Z or Company Q. The processing time was comparable using kits from Omega Bio-tek and Company Z at minimum culture volume and was lower by 2 min for Omega Bio-tek at maximum volume. The processing times shown are actual timed extractions including all pipetting, centrifugation, and bacterial culture pelleting steps. Omega Bio-tek outperformed Company Q with respect to processing time at both the culture volumes tested.
HIGH-QUALITY PLASMID DNA SUITABLE FOR SEQUENCING REACTIONS
Table 2. Plasmid DNA purified using the E.Z.N.A. FastFilter Plasmid DNA Mini Kit was used in a 5 μL Sanger sequencing reaction. The sequencing reaction was analyzed on an Applied Biosystems 3730XL. The samples had an average CRL of 994 bp and an average QV20+ of 985 with a quality score greater than 20 (≤ 1% probability of error in base calling).
VERSATILITY USING DIFFERENT PLASMID TYPES AND CULTURE MEDIA
Figure 2. E.Z.N.A.® FastFilter Plasmid DNA Mini Kit is capable of handling diverse sample input conditions ranging from plasmid types, culture medium as well as input culture volume. The plasmid yield may vary based on these conditions.
COMPLETE DIGESTION WITH VARIOUS RESTRICTION ENZYMES
Figure 3. pGEM Plasmid DNA from overnight bacterial cultures was purified using the E.Z.N.A. FastFilter Plasmid DNA Mini Kit and analyzed by restriction digestion. Complete digestion was observed for the various restriction enzymes tested.
HIGH-QUALITY, SUPERCOILED PLASMID DNA
Figure 4. Plasmid DNA Quality Assessment using kits from Omega Bio-tek or Company Q or Company Z. No genomic DNA was present on the agarose gel.
Product Documents:
Product Flyer
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