Gram Stain Kit
Details
The Gram Stain Kit is intended for the demonstration and differentiation of Gram-positive and Gram-negative bacteria.
Gram Positive Bacteria: Blue
Gram Negative Bacteria: Red
Other Tissue: Yellow
Nuclei: Red
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue: Any well fixed paraffin embedded tissue section
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Flood slide with Gentian Violet Solution and incubate for 1 minute.
3. Rinse slide in distilled water to remove excess stain.
4. Flood slide with Lugol’s Iodine Solution and incubate for 1 minute.
5. Rinse slide in running tap water to remove excess Iodine.
6. Place slide in Gram’s Decolorizer until color no longer bleeds off section.
7. Rinse slide quickly in running tap water.
8. Flood slide with Carbol Fuchsin and incubate for 1-2 minutes.
9. Rinse slide quickly in running tap water to remove excess stain.
10. Apply Tartrazine Solution and incubate for 15 seconds.
11. Dehydrate slide quickly in 3 changes of absolute alcohol.
12. Clear in 2 changes of xylene or xylene substitute, and mount in synthetic resin
Click for Instructions for Use
Gram Positive Bacteria: Blue
Gram Negative Bacteria: Red
Other Tissue: Yellow
Nuclei: Red
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue: Any well fixed paraffin embedded tissue section
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Flood slide with Gentian Violet Solution and incubate for 1 minute.
3. Rinse slide in distilled water to remove excess stain.
4. Flood slide with Lugol’s Iodine Solution and incubate for 1 minute.
5. Rinse slide in running tap water to remove excess Iodine.
6. Place slide in Gram’s Decolorizer until color no longer bleeds off section.
7. Rinse slide quickly in running tap water.
8. Flood slide with Carbol Fuchsin and incubate for 1-2 minutes.
9. Rinse slide quickly in running tap water to remove excess stain.
10. Apply Tartrazine Solution and incubate for 15 seconds.
11. Dehydrate slide quickly in 3 changes of absolute alcohol.
12. Clear in 2 changes of xylene or xylene substitute, and mount in synthetic resin
Click for Instructions for Use
| Components | Size | SDS Links |
|---|---|---|
| Gentian Violet Solution | 125 mL | View SDS |
| Lugol’s Iodine Solution | 125 mL | View SDS |
| Gram’s Decolorizer Solution | 125 mL | View SDS |
| Carbol Fuchsin Solution | 125 mL | View SDS |
| Tartrazine Solution | 125 mL | View SDS |
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