Luxol Fast Blue Stain Kit
Details
The Luxol Fast Blue Stain Kit is intended for staining myelin/myelinated axons and Nissil substance on formalin fixed, paraffin-embedded tissue as well as frozen tissue. This product is used for identifying the basic neuronal structure in brain or spinal cord sections.
Myleinated Fibers: Blue
Nissil Substance: Violet
Nerve Cells: Violet
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue:
- Cerebral Cortex
- Spinal Cord
Procedure:
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Incubate slide in Luxol Fast Blue Solution for 24 hours at room temperature or 2 hours at 60°C.
3. Rinse thoroughly in distilled water.
4. Differentiate section by dipping in Lithium Carbonate Solution (0.05%) several times (up to 20 seconds).
5. Continue differentiation by repeatedly dipping in Alcohol, Reagent (70%) until gray-matter is colorless and white-matter remains blue.
6. Rinse slide in distilled water.
7. Incubate slide in Cresyl Echt Violet (0.1%) for 2-5 minutes.
8. Rinse quickly in 1 change of distilled water.
9. Dehydrate quickly in 3 changes of absolute alcohol.
10. Clear as desired and mount in synthetic resin.
Click for Instructions for Use
Myleinated Fibers: Blue
Nissil Substance: Violet
Nerve Cells: Violet
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue:
- Cerebral Cortex
- Spinal Cord
Procedure:
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Incubate slide in Luxol Fast Blue Solution for 24 hours at room temperature or 2 hours at 60°C.
3. Rinse thoroughly in distilled water.
4. Differentiate section by dipping in Lithium Carbonate Solution (0.05%) several times (up to 20 seconds).
5. Continue differentiation by repeatedly dipping in Alcohol, Reagent (70%) until gray-matter is colorless and white-matter remains blue.
6. Rinse slide in distilled water.
7. Incubate slide in Cresyl Echt Violet (0.1%) for 2-5 minutes.
8. Rinse quickly in 1 change of distilled water.
9. Dehydrate quickly in 3 changes of absolute alcohol.
10. Clear as desired and mount in synthetic resin.
Click for Instructions for Use
| Components | Size | SDS Links |
|---|---|---|
| Cresyl Echt Violet Solution | 125 mL | |
| Luxol Fast Blue Solution | 125 mL | View SDS |
| Lithium Carbonate Solution (0.05%) | 500 mL | View SDS |
| Alcohol, Reagent (70%) | 500 mL | View SDS |
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