Mag-Bind® TotalPure NGS Magnetic Beads
Details
Mag-Bind® TotalPure NGS offers an easy-to-use, reliable solution for the purification of both DNA and RNA for next-generation sequencing workflows with high recovery rates. Mag-Bind® TotalPure NGS is capable of selectively binding fragments depending on the reagent-to-sample ratio used, giving the user flexibility to perform left, right, or double sized selection. This product is designed for both manual and fully automated purification of DNA and RNA samples, as well as for the purification of PCR products. The system combines Omega Bio-tek's proprietary chemistries with reversible nucleic acid-binding properties of magnetic beads to selectively bind fragments and eliminate nucleotides, primers, and small, non-targeted products such as primer-dimers. Purified DNA and RNA is suitable for a variety of downstream applications such as NGS library preparation, microarrays, automated fluorescent sequencing and restriction enzyme digestion.
Features:
- No protocol change against major competitor
- NGS: Double or single-sided size selection
- DNA cleanup: PCR cleanup
- RNA cleanup: cDNA or RNA purification
- Manual or adaptable to most open-ended liquid handlers
- Significant cost savings
- 96- or 384-well formats
Specifications:
Size: 5mL, 50mL, or 500mL
Downstream application: Cloning, NGS, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
Elution volume: 15 µL or above
Starting material: DNA or RNA: PCR products, gDNA, cDNA
Starting amount: Scalable
DNA recovered: >90% recovery for DNA >100 bp
Processing mode: Automated, manual
Throughput: 96-384 samples per run
DNA binding technology: Magnetic beads
Storage: 2°C - 8°C
Special note: Size selection by varying beads ratio
Protocols are available for the following automated platforms:
• Hamilton Microlab® STAR
• Hamilton Microlab® NIMBUS
• KingFisher™, BioSprint®, and MagMAX® 96
Mag-Bind TotalPure NGS from Omega Bio-tek performs excellent double-side size selection.
Figure 1. Electropherogram overlay of the double-sided size selection on sheared gDNA at 0.8x/0.7x ratio set using Omega Bio-tek’s Mag-Bind® TotalPure NGS and a comparable kit from Company A following manufacturer’s recommended protocols.
Mag-Bind TotalPure NGS from Omega Bio-tek has excellent recovery of targeted DNA fragments.
Figure 2. 10 µL of 50 bp ladder was purified with Omega Bio-tek’s Mag-Bind® TotalPure NGS and a comparable product from Company A following manufacturer’s recommended protocols.
Mag-Bind TotalPure NGS from Omega Bio-tek performs excellent RNA clean-up in a wide concentration range.
Figure 3. 10 µL of RNA at 50 ng/µL and 5 ng/µL was cleaned up with Omega Bio-tek’s Mag-Bind® TotalPure NGS following manufacturer’s recommended protocols. The RNA was eluted in 20 µL. Recovery rates were 85-92% respectively.
Mag-Bind TotalPure NGS from Omega Bio-tek performs excellent double-sided size selection on DNA from NGS library prep.
Figure 4. Next-generation sequencing libraries prepared from 350 ng sheared genomic DNA using Kapa Biosystem’s HyperPrep Kits (KK8504) and Omega Bio-tek’s Mag-Bind® TotalPure NGS and a comparable product from Company A on the Hamilton Microlab® STAR™. Mag-Bind® TotalPure NGS was used for 2 clean up step (0.8x and 1.0x) following Kapa Biosystems’ recommended protocol for clean up.
Product Documents:
Product Instructions
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