P.T.A.H. Stain Kit for Microwave
Details
The P.T.A.H. Stain Kit for Microwave is intended for use in the histological visualization of collagen, striated muscle and glial fibers without using Zenker’s Fixative with Mercuric Chloride as a mordant. This kit may be used on formalin-fixed, paraffin-embedded, or frozen sections.
Fibrin, Striated Muscle, Glial Fibers: Blue
Collagen: Brownish/Red
Nuclei: Blue
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue: Striated Muscle
Equipment Needed: 500 Watt Microwave Oven
Procedure:
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Place slide to fresh distilled water for 1 minute.
3. Pour 50 ml of Zinc Chloride Solution (10%) into plastic coplin jar and heat in microwave for 20 seconds on high power. Remove jar and stir solution to equalize temperature. Return coplin jar to microwave and heat for 10 seconds on high power. Remove jar and stir solution to equalize temperature.
4. Place slide in coplin jar and incubate for 15 minutes.
5. Rinse slide in running tap water for 1 minute.
6. Rinse in distilled water for 1 minute.
7. Place slide in 25 ml Ferric Ammonium Sulfate Aqueous Solution, heat in microwave for 15 seconds on high power and incubate for 2 minutes.
8. Rinse slide in running tap water for 2 minutes.
9. Rinse in distilled water for 1 minute.
10. Heat 25 ml Phosphotungstic Acid Hematoxylin Solution in microwave for 20 seconds on high power. Remove and agitate to equalize temperature of solution. Place slide in stain, agitate and incubate for 15 minutes. Reheat solution for 10 seconds on high power, agitate and incubate for another 15 minutes.
11. Differentiate section in 95% Reagent Alcohol. Check section using microscope for proper differentiation.
12. Dehydrate in 3 changes of Absolute Alcohol.
13. Clear in 3 changes of fresh Xylene or Xylene Substitute, and mount in synthetic resin
Click for Instructions for Use
Fibrin, Striated Muscle, Glial Fibers: Blue
Collagen: Brownish/Red
Nuclei: Blue
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue: Striated Muscle
Equipment Needed: 500 Watt Microwave Oven
Procedure:
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Place slide to fresh distilled water for 1 minute.
3. Pour 50 ml of Zinc Chloride Solution (10%) into plastic coplin jar and heat in microwave for 20 seconds on high power. Remove jar and stir solution to equalize temperature. Return coplin jar to microwave and heat for 10 seconds on high power. Remove jar and stir solution to equalize temperature.
4. Place slide in coplin jar and incubate for 15 minutes.
5. Rinse slide in running tap water for 1 minute.
6. Rinse in distilled water for 1 minute.
7. Place slide in 25 ml Ferric Ammonium Sulfate Aqueous Solution, heat in microwave for 15 seconds on high power and incubate for 2 minutes.
8. Rinse slide in running tap water for 2 minutes.
9. Rinse in distilled water for 1 minute.
10. Heat 25 ml Phosphotungstic Acid Hematoxylin Solution in microwave for 20 seconds on high power. Remove and agitate to equalize temperature of solution. Place slide in stain, agitate and incubate for 15 minutes. Reheat solution for 10 seconds on high power, agitate and incubate for another 15 minutes.
11. Differentiate section in 95% Reagent Alcohol. Check section using microscope for proper differentiation.
12. Dehydrate in 3 changes of Absolute Alcohol.
13. Clear in 3 changes of fresh Xylene or Xylene Substitute, and mount in synthetic resin
Click for Instructions for Use
| Components | Size | SDS Links |
|---|---|---|
| Zinc Chloride Solution (10%) | 500 mL | View SDS |
| Ferric Ammonium Sulfate | 125 mL | View SDS |
| P.T.AH. Solution | 125 mL | View SDS |
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