New fluorophors have been developed to replace the traditional method of nucleic acid detection with toxic Ethidium bromide. They also eliminate the use of DNA damaging UV light, as they are visualized with blue light. The SmartBlue Transilluminator is the perfect partner for these new stains. It emits blue light at a wavelength of 465nm, perfect for excitation of most green fluorophors, including SmartGlow™, as well as many protein stains. Exposing DNA to this wavelength does not cause damage, and results in a much higher downstream cloning efficiency as compared to even short UV light exposures. In addition, the visible blue light is not damaging to the skin and is less harmful to the eyes.*
The SmartBlue transilluminator requires no special shielding, however, the light does need to be filtered for visualization of the dyes. The amber cover, which rests above the viewing surface and gel, filters out the blue light, allowing the bands to be seen clearly. The cover can be placed at a 60º angle to provide easy access to the gel for band excision. For documentation, the filter cover is easily removed completely.
Engineered diffusers and filters provide an extremely evenly lit viewing surface. Even in ambient light, bands of DNA can clearly be seen in gels. Images obtained using the SmartBlue Transilluminator are brighter and more balanced than those obtained using epi-illuminators which light from the sides.
An array of super bright LEDs with a long, 30,000 hour service life provide the light source for the SmartBlue Transilluminator. Unlike those in a UV transilluminator, the filters in the SmartBlue will not solarize and degrade in performance over time. Gel bands can be excised directly on the scratch resistant, glassviewing surface. To save energy, the power switch includes an automatic 5 minute shutoff.
*Due to the high intensity of the LED lights, it is recommended not to stare directly at the viewing surface without the amber cover in place
- Light source: High intensity LEDs
- Filter Cover: Amber, for filtering of blue light
- Exterior Dimensions: 12.8 x 8.5 x 1.9 in. (30.5 x 21.8 x 5 cm)
- Output wavelength: Peak at 465nm
- Viewing surface: 6.6 x 4.75 in. (17 x 12 cm)
- Weight: 2.6 lbs. (1.2 kg)
- Electrical: 100 to 240V, 50-60HZ