Steiner Stain Kit (For Spirochetes)
Details
The Steiner Stain Kit (For Spirochetes) is designed for demonstrating Fungi, Helicobacter Pylori, Legionella pneumophila, and Spirochete infected tissue. Kit may be used on formalin fixed, paraffin-embedded tissue as well as frozen sections.
Spirochetes: Black to Brown
Helicobactor Pylori: Black to Brown
Fungi: Black to Brown
Legionella pneumophila: Black to Brown
Background: Yellow to Tan
Uses/Limitations:
- For In-Vitro Diagnostic use only.
- Histological applications.
- Do not use past expiration date.
- Use caution when handling these reagents.
Control Tissue: Helicobacter Pylori infected stomach.
Prepare Reducing Solution at Step #10 of this Procedure:
Combine:
Procedure (Standard):
1. Preheat Water Bath to 70° Centigrade.
2. Deparaffinize sections if necessary and hydrate to distilled water.
3. Incubate slide in Oxidizer Solution for 20 minutes.
4. Rinse thoroughly in distilled water.
Note: Place 20 ml of Silver Nitrate Solution (0.2%) in water bath to preheat.
5. Incubate slide in Zinc Formalin Solution for 5 minutes.
6. Rinse thoroughly in distilled water.
7. Incubate slide in preheated Silver Nitrate Solution (0.2%) for 5 minutes at 70° Centigrade. (Note: Discard solution after this step)
8. Rinse slide thoroughly in distilled water.
9. Dehydrate slide in 2 changes of Absolute Alcohol.
Note: Prepare Reducing Solution (above) and place in water bath to preheat.
10. Incubate slide in Gum Mastic Solution for 3 minutes.
11. Air dry slide for 1 minute or until gum mastic is completely dry.
12. Incubate slide in preheated Reducing Solution for 10-15 minutes or until section is tan to brown at 70° Centigrade. (Note: Discard solution after this step)
13. Rinse slide quickly in distilled water.
14. Dehydrate quickly in 3 changes of absolute alcohol.
15. Clear, and mount in synthetic resin.
Procedure (Microwave):
Prepare Reducing Solution at Step #10 of this Procedure:
Combine:
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Incubate slide in Oxidizer Solution for 20 minutes.
3. Rinse thoroughly in distilled water.
4. Incubate slide in Zinc Formalin Solution for 5 minutes.
5. Rinse thoroughly in distilled water.
Note: In a loosely capped Slide Jar heat 20ml of Silver Nitrate Solution (0.2%) in a microwave oven for 10 seconds at full power. Repeat as needed until solution is hot, but do not allow solution to boil. Remove Slide Jar from microwave, tighten cap and agitate to equalize temperature.
6. Incubate slide in hot Silver Nitrate Solution (0.2%) for 2 minutes with occasional agitation. (Note: Discard solution after this step)
7. Rinse slide thoroughly in distilled water.
8. Dehydrate slide in 2 changes of Absolute Alcohol.
9. Incubate slide in Gum Mastic Solution for 3 minutes.
Note: Prepare Reducing Solution (above).
10. Air dry slide for 1 minute or until gum mastic is completely dry.
11. In a loosely capped Slide Jar heat 20ml of Reducing Solution in a microwave oven for 10 seconds at full power. Repeat as needed until solution is hot, but do not allow solution to boil. Remove Slide Jar from microwave, tighten cap and agitate to equalize temperature.
12. Place slide in loosely capped Slide Jar and return to microwave. As before heat Slide Jar containing slide for 10 seconds at full power. Repeat as needed until solution is hot, but do not allow solution to boil. Incubate slide in hot Reducing Solution for 3 minutes and then reheat again at full power until solution is hot. Incubate slide for an additional 2-3 minutes or until section is tan to brown. (Note: Discard solution after this step)
13. Rinse slide quickly in distilled water.
14. Dehydrate quickly in 3 changes of absolute alcohol.
15. Clear, and mount in synthetic resin.
Spirochetes: Black to Brown
Helicobactor Pylori: Black to Brown
Fungi: Black to Brown
Legionella pneumophila: Black to Brown
Background: Yellow to Tan
Uses/Limitations:
- For In-Vitro Diagnostic use only.
- Histological applications.
- Do not use past expiration date.
- Use caution when handling these reagents.
Control Tissue: Helicobacter Pylori infected stomach.
Prepare Reducing Solution at Step #10 of this Procedure:
Combine:
25ml 1% Hydroquinone (0.25gm Hydroquinone in 25ml Distilled Water)Then Add:
15ml Gum Mastic Solution (2.5%)
Mix thoroughly and filter through medium filter paper.
6 Drops (240?l) Silver Nitrate Solution (1%)
Mix thoroughly.
Procedure (Standard):
1. Preheat Water Bath to 70° Centigrade.
2. Deparaffinize sections if necessary and hydrate to distilled water.
3. Incubate slide in Oxidizer Solution for 20 minutes.
4. Rinse thoroughly in distilled water.
Note: Place 20 ml of Silver Nitrate Solution (0.2%) in water bath to preheat.
5. Incubate slide in Zinc Formalin Solution for 5 minutes.
6. Rinse thoroughly in distilled water.
7. Incubate slide in preheated Silver Nitrate Solution (0.2%) for 5 minutes at 70° Centigrade. (Note: Discard solution after this step)
8. Rinse slide thoroughly in distilled water.
9. Dehydrate slide in 2 changes of Absolute Alcohol.
Note: Prepare Reducing Solution (above) and place in water bath to preheat.
10. Incubate slide in Gum Mastic Solution for 3 minutes.
11. Air dry slide for 1 minute or until gum mastic is completely dry.
12. Incubate slide in preheated Reducing Solution for 10-15 minutes or until section is tan to brown at 70° Centigrade. (Note: Discard solution after this step)
13. Rinse slide quickly in distilled water.
14. Dehydrate quickly in 3 changes of absolute alcohol.
15. Clear, and mount in synthetic resin.
Procedure (Microwave):
Prepare Reducing Solution at Step #10 of this Procedure:
Combine:
25ml 1% Hydroquinone (0.25gm Hydroquinone in 25ml Distilled Water)Then Add:
15ml Gum Mastic Solution (2.5%)
Mix thoroughly and filter through medium filter paper.
6 Drops (240?l) Silver Nitrate Solution (1%)
Mix thoroughly.
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Incubate slide in Oxidizer Solution for 20 minutes.
3. Rinse thoroughly in distilled water.
4. Incubate slide in Zinc Formalin Solution for 5 minutes.
5. Rinse thoroughly in distilled water.
Note: In a loosely capped Slide Jar heat 20ml of Silver Nitrate Solution (0.2%) in a microwave oven for 10 seconds at full power. Repeat as needed until solution is hot, but do not allow solution to boil. Remove Slide Jar from microwave, tighten cap and agitate to equalize temperature.
6. Incubate slide in hot Silver Nitrate Solution (0.2%) for 2 minutes with occasional agitation. (Note: Discard solution after this step)
7. Rinse slide thoroughly in distilled water.
8. Dehydrate slide in 2 changes of Absolute Alcohol.
9. Incubate slide in Gum Mastic Solution for 3 minutes.
Note: Prepare Reducing Solution (above).
10. Air dry slide for 1 minute or until gum mastic is completely dry.
11. In a loosely capped Slide Jar heat 20ml of Reducing Solution in a microwave oven for 10 seconds at full power. Repeat as needed until solution is hot, but do not allow solution to boil. Remove Slide Jar from microwave, tighten cap and agitate to equalize temperature.
12. Place slide in loosely capped Slide Jar and return to microwave. As before heat Slide Jar containing slide for 10 seconds at full power. Repeat as needed until solution is hot, but do not allow solution to boil. Incubate slide in hot Reducing Solution for 3 minutes and then reheat again at full power until solution is hot. Incubate slide for an additional 2-3 minutes or until section is tan to brown. (Note: Discard solution after this step)
13. Rinse slide quickly in distilled water.
14. Dehydrate quickly in 3 changes of absolute alcohol.
15. Clear, and mount in synthetic resin.
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