E.Z.N.A.® Gel Extraction Kit
Details
The E.Z.N.A.® Gel Extraction kit uses the proprietary HiBind® spin-column technology to purify DNA fragments ranging from 100 bp to 10 kb from all grades of agarose gels with high recovery (> 90%). The kit uses a specialized binding buffer system that not only dissolves the gel slice and binds to the spin column but also includes a pH indicator for a visual representation of optimal pH for DNA binding. The bind step is followed by three rapid wash steps and DNA is eluted with deionized water or elution buffer. Purified DNA is ready for a variety of downstream applications such as ligations, PCR amplification, restriction enzyme digestion, cloning, and various labeling reactions.
Features:
- Rapid, DNA recovery from an agarose gel in 15 min
- Specialized buffer system, visual determination of optimum DNA binding for higher yields
- Safe, no phenol/chloroform extractions
- Versatile, spin and vacuum formats available
- High-quality, DNA is suitable for a variety of downstream applications
Specifications:
Prep count: 5, 50, or 200 preps
Downstream application: Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
Elution volume: 30-50 µL
Starting material: Agrose gel slice
Starting amount: Up to 25 µg DNA
DNA recovered: >90% recovery, 70 bp to 20 kb
Processing mode: Manual, centrifugation/vacuum
Throughout: 1-12
DNA binding technology: Silica mini spin column
Blinding capacity: 25 μg
Processing time: <10 minutes
Electrophoretic Analysis of recovered DNA from an agarose gel compared to the control
Figure 1. DNA ladder was run on a 2% agarose gel and 4 different fragment sizes (200 bp, 500 bp, 1 kb, and 5 kb) were recovered using Omega Bio-tek’s E.Z.N.A.® Gel Extraction Kit and a comparable kit from Company Q following manufacturer’s recommended protocols. DNA was analyzed on a 2% TBE agarose gel with the respective companies eluate being compared to the original amount used in the gel extraction procedure. M: size marker; C: control; O: Omega Bio-tek; Q: company Q.
Comparison of DNA fragment recovery from an agarose gel
Figure 2. DNA was purified from an agarose gel using Omega Bio-tek’s E.Z.N.A.® Gel Extraction Kit and a comparable kit from Company Q.
Product Documents:
Product Flyer
Features:
- Rapid, DNA recovery from an agarose gel in 15 min
- Specialized buffer system, visual determination of optimum DNA binding for higher yields
- Safe, no phenol/chloroform extractions
- Versatile, spin and vacuum formats available
- High-quality, DNA is suitable for a variety of downstream applications
Specifications:
Prep count: 5, 50, or 200 preps
Downstream application: Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
Elution volume: 30-50 µL
Starting material: Agrose gel slice
Starting amount: Up to 25 µg DNA
DNA recovered: >90% recovery, 70 bp to 20 kb
Processing mode: Manual, centrifugation/vacuum
Throughout: 1-12
DNA binding technology: Silica mini spin column
Blinding capacity: 25 μg
Processing time: <10 minutes
Electrophoretic Analysis of recovered DNA from an agarose gel compared to the control
Figure 1. DNA ladder was run on a 2% agarose gel and 4 different fragment sizes (200 bp, 500 bp, 1 kb, and 5 kb) were recovered using Omega Bio-tek’s E.Z.N.A.® Gel Extraction Kit and a comparable kit from Company Q following manufacturer’s recommended protocols. DNA was analyzed on a 2% TBE agarose gel with the respective companies eluate being compared to the original amount used in the gel extraction procedure. M: size marker; C: control; O: Omega Bio-tek; Q: company Q.
Comparison of DNA fragment recovery from an agarose gel
Figure 2. DNA was purified from an agarose gel using Omega Bio-tek’s E.Z.N.A.® Gel Extraction Kit and a comparable kit from Company Q.
Product Documents:
Product Flyer
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