E.Z.N.A.® Micro RNA Kit
Details
The E.Z.N.A.® Micro RNA Kit combines the reversible binding properties of the HiBind® matrix, a new silica-based material, with the highly efficient lysis ability of RNA-Solv® to extract micro and large (>200 nt) RNA from a wide variety of starting materials. Micro and large RNA are separated to allow for quantification into downstream applications.
The system uses RNA-Solv® to efficiently lyse cells and protect RNA from degradation. Following a chloroform separation step, RNA is applied to a HiBind® RNA column where large RNA binds. The flow-through is retained and applied to a HiBind® Micro RNA column to bind small RNA.
Features:
- Combines the reversible binding properties of the HiBind® matrix and a new silica-based material
- Micro and large RNA are separated to allow for quantification into downstream applications
Specifications:
Prep count: 50 preps
Downstream application: RTPCR*, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation
Elution volume: 15-30 μL for miRNA; 40-70 μL for >200 nt
Starting material: Cultured eukaryotic cells or bacteria, or from animal, plant, or fungal tissues
Starting amount: Up to 100 mg
RNA yield: Up to 50 µg
Processing mode: 1-24
RNA binding technology: Silica technology
Binding capacity: 100 µg for each
MicroRNA was purified using the E.Z.N.A.® Micro RNA Kit
Figure 1. Purified large RNAs and miRNA from tissue samples was isolated with the E.Z.N.A.® miRNA Isolation Kit. RNA was extracted from samples as follows: sample 1 from fish liver, sample 2 from frog liver. Large RNA (5% of total purified RNA) was analyzed on a 1% agarose gel. miRNA (5% of total purified RNA) was analyzed on a 3% agarose gel to demonstrate yield and quality of the RNA.
Product Documents:
Product Flyer
The system uses RNA-Solv® to efficiently lyse cells and protect RNA from degradation. Following a chloroform separation step, RNA is applied to a HiBind® RNA column where large RNA binds. The flow-through is retained and applied to a HiBind® Micro RNA column to bind small RNA.
Features:
- Combines the reversible binding properties of the HiBind® matrix and a new silica-based material
- Micro and large RNA are separated to allow for quantification into downstream applications
Specifications:
Prep count: 50 preps
Downstream application: RTPCR*, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation
Elution volume: 15-30 μL for miRNA; 40-70 μL for >200 nt
Starting material: Cultured eukaryotic cells or bacteria, or from animal, plant, or fungal tissues
Starting amount: Up to 100 mg
RNA yield: Up to 50 µg
Processing mode: 1-24
RNA binding technology: Silica technology
Binding capacity: 100 µg for each
MicroRNA was purified using the E.Z.N.A.® Micro RNA Kit
Figure 1. Purified large RNAs and miRNA from tissue samples was isolated with the E.Z.N.A.® miRNA Isolation Kit. RNA was extracted from samples as follows: sample 1 from fish liver, sample 2 from frog liver. Large RNA (5% of total purified RNA) was analyzed on a 1% agarose gel. miRNA (5% of total purified RNA) was analyzed on a 3% agarose gel to demonstrate yield and quality of the RNA.
Product Documents:
Product Flyer
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