E.Z.N.A.® MicroElute RNA Clean Up Kit
Details
The E.Z.N.A.® MicroElute RNA Clean Up Kit is designed to purify and concentrate RNA (from 50 µg down to picogram amounts) from enzymatic reactions or for desalting RNA samples (e.g. following the phenol extraction and ethanol precipitation). The procedure uses lower elution volumes, making it well suited for applications requiring highly concentrated RNA samples.
Features:
- Recovery of RNA from enzymatic reactions in 15 minutes
- No organic extractions
- Clean up RNA isolated by others methods (e.g. crude preps from organic extraction methods)
- Concentrates small amounts of RNA to 10-15µL
Specifications:
Prep count: 5 or 50 preps
Downstream application: Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
Elution volume: 10-15 µL
Starting material: Enzymaic reaction
Starting amount: Up to 10 µg RNA
DNA recovered: >90% recovery, RNA > 200 bases
Processing mode: Manual, centrifugation
Throughput: 1
DNA binding technology: Silica MicroElute spin column
Binding capacity: 10 µg
Processing time: <15 minutes
Special note: Low elution volume, suitable for RNA desalting too
RNA was intact after being purified using the MicroElute RNA Clean Up Kit
Figure 1. Total RNA purified using the MicroElute RNA Clean Up Kit. Total RNA was purified from 15 µL RNA original solution and eluted in 15 µL elution buffer. Total RNA (2 µL) was analyzed on a 1% agarose gel. Lanes 1-4: after purification, C: before purification.
Product Documents:
Product Flyer
Features:
- Recovery of RNA from enzymatic reactions in 15 minutes
- No organic extractions
- Clean up RNA isolated by others methods (e.g. crude preps from organic extraction methods)
- Concentrates small amounts of RNA to 10-15µL
Specifications:
Prep count: 5 or 50 preps
Downstream application: Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
Elution volume: 10-15 µL
Starting material: Enzymaic reaction
Starting amount: Up to 10 µg RNA
DNA recovered: >90% recovery, RNA > 200 bases
Processing mode: Manual, centrifugation
Throughput: 1
DNA binding technology: Silica MicroElute spin column
Binding capacity: 10 µg
Processing time: <15 minutes
Special note: Low elution volume, suitable for RNA desalting too
RNA was intact after being purified using the MicroElute RNA Clean Up Kit
Figure 1. Total RNA purified using the MicroElute RNA Clean Up Kit. Total RNA was purified from 15 µL RNA original solution and eluted in 15 µL elution buffer. Total RNA (2 µL) was analyzed on a 1% agarose gel. Lanes 1-4: after purification, C: before purification.
Product Documents:
Product Flyer
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