E.Z.N.A.® Total RNA Kit I
Details
The E.Z.N.A.® Total RNA Kit I provides a straightforward and rapid method for the isolation of up to 100 µg of total RNA from cultured eukaryotic cells and soft tissues. Multiple samples of up to 1 x 107 eukaryotic cells or 30 mg of tissue can be processed in parallel in fewer than 20 minutes. Purified RNA can be used in many downstream applications such as RT-PCR, Northern blotting, nuclease protection assay, and in vitro translation.
Features:
- Rapid, 20 minutes or less
- Safe, no phenol/chloroform extractions
- Versatile, spin and vacuum formats available
- High quality, purified RNA suitable for a variety of downstream applications
Specifications:
Prep count: 5, 50 or 200 preps
Downstream application: PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution volume: 40-70 μL
Starting material: Cultured eukaryotic cells and soft tissues
Starting amount: < 1 x 107 cells or 30 mg tissue
RNA yield: Up to 100 µg
Processing mode: Manual, centrifugation/vacuum
Throughput: 1-24
RNA binding technology: Silica mini spin column
Binding capacity: 100 µg
Expected RNA yields from mouse tissue samples and cultured cell types with E.Z.N.A.® Total RNA Kit I.
Figure 1. Expected RNA yields from mouse tissue samples and cultured cell types with E.Z.N.A.® Total RNA Kit I.
RNA yields from HEK-293 cells using E.Z.N.A.® Total RNA Kit I.
Figure 2. Total RNA was isolated from 2.5×106 HEK-293 cultured cells with Omega Bio-tek’s E.Z.N.A.® Total RNA Kit I with an elution volume of 50 µL. RNA concentrations were quantified with Thermo Scientific’s NanoDrop® 2000c. RNA quality was assessed with the Agilent TapeStation® 2200.
Product Documents:
Product Flyer
Features:
- Rapid, 20 minutes or less
- Safe, no phenol/chloroform extractions
- Versatile, spin and vacuum formats available
- High quality, purified RNA suitable for a variety of downstream applications
Specifications:
Prep count: 5, 50 or 200 preps
Downstream application: PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution volume: 40-70 μL
Starting material: Cultured eukaryotic cells and soft tissues
Starting amount: < 1 x 107 cells or 30 mg tissue
RNA yield: Up to 100 µg
Processing mode: Manual, centrifugation/vacuum
Throughput: 1-24
RNA binding technology: Silica mini spin column
Binding capacity: 100 µg
Expected RNA yields from mouse tissue samples and cultured cell types with E.Z.N.A.® Total RNA Kit I.
Figure 1. Expected RNA yields from mouse tissue samples and cultured cell types with E.Z.N.A.® Total RNA Kit I.
RNA yields from HEK-293 cells using E.Z.N.A.® Total RNA Kit I.
Figure 2. Total RNA was isolated from 2.5×106 HEK-293 cultured cells with Omega Bio-tek’s E.Z.N.A.® Total RNA Kit I with an elution volume of 50 µL. RNA concentrations were quantified with Thermo Scientific’s NanoDrop® 2000c. RNA quality was assessed with the Agilent TapeStation® 2200.
Product Documents:
Product Flyer
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