E.Z.N.A.® Viral RNA Kit
Details
The E.Z.N.A.® Viral RNA Kit is designed for the isolation of viral DNA and viral RNA from cell-free fluids such as plasma, serum, urine and cell culture supernatant. The procedure completely removes contaminants and enzyme inhibitors making viral RNA isolation fast, convenient and reliable. The kit is also suitable for the isolation of total RNA from cultured cells, tissues and bacteria. RNA purified using the E.Z.N.A.® Viral RNA method is ready for all downstream applications such as RT-PCR.
Features:
- Designed for the isolation of viral DNA and viral RNA
- Completely removes contaminants and enzyme inhibitors
- Also suitable for the isolation of total RNA
- Ready for all downstream applications such as RT-PCR
Specifications:
Prep quantity: 5, 50, or 200 preps
Starting amount: 150 µL
Starting material: Cell free fluids such as plasma, serum, urine and cell culture supernatant
Elution volume: 20-50 µL
Technology: HiBind® RNA Mini Column
Processing mode: Manual, centrifugation/vacuum
Throughput: 1-24
Viral RNA extracted using E.Z.N.A.® Viral RNA Kit was PCR inhibitor free
Figure 1. 25 µL of Zeptomatrix Influenza A/B Positive Control was spiked into 125 µL serum samples and then Viral RNA was extracted using the E.Z.N.A.® Viral RNA Kit . 2 µL of Eluted RNA was used as a template in a 20 µL SYBR® qPCR reaction. C: inhibitor-free control; T: RNA sample extracted with kit. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template RNA in free of inhibitors.
Product Documents:
Product Flyer
Features:
- Designed for the isolation of viral DNA and viral RNA
- Completely removes contaminants and enzyme inhibitors
- Also suitable for the isolation of total RNA
- Ready for all downstream applications such as RT-PCR
Specifications:
Prep quantity: 5, 50, or 200 preps
Starting amount: 150 µL
Starting material: Cell free fluids such as plasma, serum, urine and cell culture supernatant
Elution volume: 20-50 µL
Technology: HiBind® RNA Mini Column
Processing mode: Manual, centrifugation/vacuum
Throughput: 1-24
Viral RNA extracted using E.Z.N.A.® Viral RNA Kit was PCR inhibitor free
Figure 1. 25 µL of Zeptomatrix Influenza A/B Positive Control was spiked into 125 µL serum samples and then Viral RNA was extracted using the E.Z.N.A.® Viral RNA Kit . 2 µL of Eluted RNA was used as a template in a 20 µL SYBR® qPCR reaction. C: inhibitor-free control; T: RNA sample extracted with kit. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template RNA in free of inhibitors.
Product Documents:
Product Flyer
Ratings & Reviews
No reviews available
Be the first to Write a Review