Gill #1 Hematoxylin

Used with hematoxylin and eosin staining. Our Gill Hematoxylin reagents are progressive nuclear stains.

Gill #1 is used for routine cytology staining to accompany EA-36 or EA-50 (for gynecological), or EA-65 (for non-gynecological) and OG-6. This single strength hematoxylin formulation optimally stains gynecological and non-gynecological specimens alike. Gill hematoxylin is a general purpose progressive type nuclear stain and presented in 3 strengths, Gill 1X, 2X, and 3X. Cell blocks are usually stained using Gill 1X Hematoxylin accompanied with Eosin to demonstrate the routine H&E stain for cytology analysis. The Gill 2X can be used when a darker nuclear stain is desired.

Features:
- Progressive nuclear stains
- Used for routine cytology staining to accompany EA-36 or EA-50 (for gynecological), or EA-65 (for non-gynecological) and OG-6
- Presented in 3 strengths

Packaging options:
ES703-16: 16oz

ES703: 4x16oz

ES704-1G: 1 Gallon

ES704: 4x1 Gallon

Procedure:
1. Fix cytological smears in 95% Ethanol: 15 minutes
2. Rinse gently in DI water: 30 seconds
3. Stain in hematoxylin solution, Gill 1X or Gill 2X: 1½ to 3 minutes
4. Rinse gently in DI water: 1 minute
5. Bluing Reagent: 15 to 60 seconds
6. Rinse gently in DI water: 30 seconds
7. Ethanol Solution, 95%:10 dips
8. Papanicolaou Stain OG-6: 1 to 2 minutes
9. Ethanol Solution, 95%: 10 dips
10. Papanicolaou Stain EA 50 or Papanicolaou Stain EA 65 EA: 2½ to 3 minutes
Note: Use EA 50 for gynecological samples; and EA 65 for non-gynecological samples. (the number indicates the proportion of the dyes)
11. Ethanol Solution, 95%, two changes: 10 dips each
12. Ethanol Solution, 100%, two changes: 1 minute each
13. Xylene or Xylene substitute, two changes: 1 minutes each
14. Coverslip using Acrylic Mounting Medium, and examine under microscope