Gill #2 Hematoxylin

Used for cytology and/or histology as a progressive or regressive stain depending on length of staining time. Our Gill Hematoxylin reagents are progressive nuclear stains. Formulated to avoid precipitation.

Gill 1X is used for routine cytology staining to accompany EA-36 or EA-50 (for gynecological), or EA-65 (for non-gynecological) and OG-6. This single strength hematoxylin formulation optimally stains gynecological and non-gynecological specimens alike. Gill hematoxylin is a general purpose progressive type nuclear stain and presented in 3 strengths, Gill 1X, 2X, and 3X. Cell blocks are usually stained using Gill 1X Hematoxylin accompanied with Eosin to demonstrate the routine H&E stain for cytology analysis. The Gill 2X can be used when a darker nuclear stain is desired.

Features:
- Progressive nuclear stains
- Used for routine cytology staining to accompany EA-36 or EA-50 (for gynecological), or EA-65 (for non-gynecological) and OG-6
- Presented in 3 strengths

Packaging options:
ES705-16: 16oz

ES705: 4x16oz

ES706-1G: 1 Gallon

ES706: 4x1 Gallon

Procedure:
1. Fix cytological smears in 95% Ethanol: 15 minutes
2. Rinse gently in DI water: 30 seconds
3. Stain in hematoxylin solution, Gill 1X or Gill 2X: 1½ to 3 minutes
4. Rinse gently in DI water: 1 minute
5. Bluing Reagent: 15 to 60 seconds
6. Rinse gently in DI water: 30 seconds
7. Ethanol Solution, 95%:10 dips
8. Papanicolaou Stain OG-6: 1 to 2 minutes
9. Ethanol Solution, 95%: 10 dips
10. Papanicolaou Stain EA 50 or Papanicolaou Stain EA 65 EA: 2½ to 3 minutes
Note: Use EA 50 for gynecological samples; and EA 65 for non-gynecological samples. (the number indicates the proportion of the dyes)
11. Ethanol Solution, 95%, two changes: 10 dips each
12. Ethanol Solution, 100%, two changes: 1 minute each
13. Xylene or Xylene substitute, two changes: 1 minutes each
14. Coverslip using Acrylic Mounting Medium, and examine under microscope

Product Documents:
Product Literature