Fite's Stain Kit (Leprosy and Nocardia Procedures)
Details
The Fite’s Stain Kit (For Leprosy and Nocardia) is intended for use in the histological visualization of mycobacterium Lepra bacillus and Nocardia. This kit may be used on formalin-fixed, paraffin-embedded or frozen sections.
Lerpa bacillus: Red
Nocardia: Red
Background: Blue
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue: Any well fixed paraffin embedded tissue
Lepra bacillus Procedure (Standard):
1. Deparaffinize sections in 2 changes of Xylene-Peanut Oil Solution for 12 minutes each.
2. Air dry slide for 15 minutes. Do not remove oil film. Remaining film prevents de-staining of Lepra bacillus during differentiation.
3. Rinse slide in several changes of distilled water.
4. Incubate slide in Carbol Fuchsin Solution for 15 minutes.
5. Rinse slide in several changes of distilled water.
6. Differentiate section in Acid Alcohol Solution (1%) until background is pink.
7. Rinse slide in distilled water and check by microscope for correct differentiation
8. Rinse in running tap water for 1 minute followed by 1 rinse in distilled water.
9. Dip slide 2-3 times in Methylene Blue Solution.
10. Dip slide quickly in distilled water and check by microscope for correct staining.
11. Air dry slide at room temperature.
12. Dip slide several times in Xylene or Xylene Substitute.
13. Mount in synthetic resin.
Nocardia Procedure:
Preparation of Reagents Prior to Beginning:
1. Prepare Diluted Acid Alcohol Solution by mixing 25ml of Acid Alcohol Solution (1%) with 25ml of Distilled Water.
Procedure:
1. Deparaffinize sections in 2 changes of Xylene-Peanut Oil Solution for 12 minutes each.
2. Air dry slide for 15 minutes. Do not remove oil film. Remaining film prevents de-staining of Lepra bacillus during
differentiation.
3. Rinse slide in several changes of distilled water.
4. Incubate slide in Carbol Fuchsin Solution for 15 minutes.
5. Rinse slide in several changes of distilled water.
6. Dip slide 2-3 times in Diluted Acid Alcohol Solution.
7. Rinse slide in distilled water and check by microscope for correct differentiation. Avoid decolorizing the Nocardia organism.
8. Rinse in running tap water for 1 minute followed by 1 rinse in distilled water.
9. Dip slide 2-3 times in Methylene Blue Solution.
10. Dip slide quickly in distilled water and check by microscope for correct staining.
11. Air dry slide at room temperature.
12. Dip slide several times in Xylene or Xylene Substitute.
13. Mount in synthetic resin.
Click for Instructions for Use
Lerpa bacillus: Red
Nocardia: Red
Background: Blue
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue: Any well fixed paraffin embedded tissue
Lepra bacillus Procedure (Standard):
1. Deparaffinize sections in 2 changes of Xylene-Peanut Oil Solution for 12 minutes each.
2. Air dry slide for 15 minutes. Do not remove oil film. Remaining film prevents de-staining of Lepra bacillus during differentiation.
3. Rinse slide in several changes of distilled water.
4. Incubate slide in Carbol Fuchsin Solution for 15 minutes.
5. Rinse slide in several changes of distilled water.
6. Differentiate section in Acid Alcohol Solution (1%) until background is pink.
7. Rinse slide in distilled water and check by microscope for correct differentiation
8. Rinse in running tap water for 1 minute followed by 1 rinse in distilled water.
9. Dip slide 2-3 times in Methylene Blue Solution.
10. Dip slide quickly in distilled water and check by microscope for correct staining.
11. Air dry slide at room temperature.
12. Dip slide several times in Xylene or Xylene Substitute.
13. Mount in synthetic resin.
Nocardia Procedure:
Preparation of Reagents Prior to Beginning:
1. Prepare Diluted Acid Alcohol Solution by mixing 25ml of Acid Alcohol Solution (1%) with 25ml of Distilled Water.
Procedure:
1. Deparaffinize sections in 2 changes of Xylene-Peanut Oil Solution for 12 minutes each.
2. Air dry slide for 15 minutes. Do not remove oil film. Remaining film prevents de-staining of Lepra bacillus during
differentiation.
3. Rinse slide in several changes of distilled water.
4. Incubate slide in Carbol Fuchsin Solution for 15 minutes.
5. Rinse slide in several changes of distilled water.
6. Dip slide 2-3 times in Diluted Acid Alcohol Solution.
7. Rinse slide in distilled water and check by microscope for correct differentiation. Avoid decolorizing the Nocardia organism.
8. Rinse in running tap water for 1 minute followed by 1 rinse in distilled water.
9. Dip slide 2-3 times in Methylene Blue Solution.
10. Dip slide quickly in distilled water and check by microscope for correct staining.
11. Air dry slide at room temperature.
12. Dip slide several times in Xylene or Xylene Substitute.
13. Mount in synthetic resin.
Click for Instructions for Use
| Components | Size | SDS Links |
|---|---|---|
| Xylene-Peanut Oil Solution | 125 mL | |
| Carbol Fuchsin Solution | 125 mL | View SDS |
| Acid Alcohol Solution (1%) | 500 mL | View SDS |
| Methylene Blue Solution | 125 mL |
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