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Periodic Acid Schiff (PAS) Stain Kit (Modified Lillie's)
Details
The Periodic Acid Schiff (PAS) Stain Kit (Modified Lillie's) is intended for use in histological demonstration of lymphocytes and mucopolysaccharides. The staining patterns of the lymphocytes are helpful in making therapeutic decisions in established cases of lymphocytic leukemia.The PAS reaction tissue sections are useful for the demonstration of mucopolysaccharides. PAS staining may also be used for the demonstration of fungal organisms in tissue sections.
PAS Positive Material: Magenta
Nuclei: Black/Blue
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue:
- Kidney
- Intestine
- Liver
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. If sections are Zenker-fixed, remove mercuric chloride crystals using iodine and clear with sodium thiosulfate. Rinse in running tap water.
3. Immerse slide in Periodic Acid Solution for 5 minutes (10 minutes for Kidney, skin and diastase digested liver sections).
4. Rinse slide in 4 changes of distilled water.
5. Immerse slide in Schiff’s Solution for 15 minutes (30 minutes for Kidney, skin and diastase digested liver sections).
6. Rinse slide in hot running tap water.
7. Rinse slide in distilled water.
8. Stain slide in Hematoxylin, Mayer’s for 2-3 minutes.
9. Rinse slide in running tap water for 2-3 minutes.
10. Apply Bluing Reagent for 30 seconds.
11. Rinse in distilled water.
12. Dehydrate through graded alcohols.
13. Clear, and mount in synthetic resin.
Click for Instructions for Use
PAS Positive Material: Magenta
Nuclei: Black/Blue
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue:
- Kidney
- Intestine
- Liver
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. If sections are Zenker-fixed, remove mercuric chloride crystals using iodine and clear with sodium thiosulfate. Rinse in running tap water.
3. Immerse slide in Periodic Acid Solution for 5 minutes (10 minutes for Kidney, skin and diastase digested liver sections).
4. Rinse slide in 4 changes of distilled water.
5. Immerse slide in Schiff’s Solution for 15 minutes (30 minutes for Kidney, skin and diastase digested liver sections).
6. Rinse slide in hot running tap water.
7. Rinse slide in distilled water.
8. Stain slide in Hematoxylin, Mayer’s for 2-3 minutes.
9. Rinse slide in running tap water for 2-3 minutes.
10. Apply Bluing Reagent for 30 seconds.
11. Rinse in distilled water.
12. Dehydrate through graded alcohols.
13. Clear, and mount in synthetic resin.
Click for Instructions for Use
| Components | Size | SDS Links |
|---|---|---|
| Periodic Acid Solution | 250 mL | View SDS |
| Schiff’s Solution | 250 mL | View SDS |
| Hematoxylin, Mayer’s | 125 mL | View SDS |
| Bluing Reagent | 125 mL | View SDS |
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