E.Z.N.A.® Bacterial RNA Kit
Details
The E.Z.N.A.® Bacterial RNA Kit is designed for the isolation of high-quality total RNA from a variety of bacterial strains. Up to 1 x 109 log-phase bacterial cells can be processed. This kit uses an improved lysis procedure to ensure the complete lysis of bacterial cells. Purified RNA is suitable for downstream applications such as RT-PCR and hybridization techniques.
Features:
- Designed for the isolation of high-quality total RNA from a variety of bacterial strains
- Uses an improved lysis procedure to ensure the complete lysis of bacterial cells
- Suitable for downstream applications such as RT-PCR and hybridization techniques
Specifications:
Prep count: 5 or 50 preps
Downstream application: PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution volume: 50-100 µL
Starting material: Bacteria
Starting amount: Up to 3 mL culture, 1 x 109 cells
RNA yield: up to 100 µg
Processing mode: Manual, centrifugation/vacuum
Throughput: 1-24
RNA binding technology: Silica Mini spin column
Binding capacity: 100 µg
Total RNA purified using E.Z.N.A.® Bacterial RNA Kit.
Figure 1. Total RNA purified using the E.Z.N.A.® Bacterial RNA Kit. RNA was extracted from 2 mL bacterial samples as follows: sample 1 from Pseudomonas arginosa, sample 2 from Enterococcus faecalis, and sample 3 from Staphylococcus aureus. Total RNA (10% of total purified RNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA. M: CL5000 DNA Marker.
Total RNA purified using E.Z.N.A.® Bacterial RNA Kit.
Figure 2. Total RNA purified using the E.Z.N.A.® Bacterial RNA Kit. RNA was extracted from lag phase E. coli cultures as follows: sample 1 from 0.5 mL culture, sample 2 from 1.0 mL culture, sample 3 from 2 mL culture, and sample 4 from 3 mL culture. Lanes 1-4 represent the first 100 µL elution. Total RNA (50% of total purified RNA) was analyzed on a denaturing formaldehyde agarose gel to demonstrate yield and quality of the RNA. M: CL5000 DNA Marker.
Product Documents:
Product Flyer
Features:
- Designed for the isolation of high-quality total RNA from a variety of bacterial strains
- Uses an improved lysis procedure to ensure the complete lysis of bacterial cells
- Suitable for downstream applications such as RT-PCR and hybridization techniques
Specifications:
Prep count: 5 or 50 preps
Downstream application: PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution volume: 50-100 µL
Starting material: Bacteria
Starting amount: Up to 3 mL culture, 1 x 109 cells
RNA yield: up to 100 µg
Processing mode: Manual, centrifugation/vacuum
Throughput: 1-24
RNA binding technology: Silica Mini spin column
Binding capacity: 100 µg
Total RNA purified using E.Z.N.A.® Bacterial RNA Kit.
Figure 1. Total RNA purified using the E.Z.N.A.® Bacterial RNA Kit. RNA was extracted from 2 mL bacterial samples as follows: sample 1 from Pseudomonas arginosa, sample 2 from Enterococcus faecalis, and sample 3 from Staphylococcus aureus. Total RNA (10% of total purified RNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA. M: CL5000 DNA Marker.
Total RNA purified using E.Z.N.A.® Bacterial RNA Kit.
Figure 2. Total RNA purified using the E.Z.N.A.® Bacterial RNA Kit. RNA was extracted from lag phase E. coli cultures as follows: sample 1 from 0.5 mL culture, sample 2 from 1.0 mL culture, sample 3 from 2 mL culture, and sample 4 from 3 mL culture. Lanes 1-4 represent the first 100 µL elution. Total RNA (50% of total purified RNA) was analyzed on a denaturing formaldehyde agarose gel to demonstrate yield and quality of the RNA. M: CL5000 DNA Marker.
Product Documents:
Product Flyer
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