E.Z.N.A.® Plasmid Mini Kit I, Q-Spin
Details
The E.Z.N.A.® Plasmid DNA Mini Kit I is designed to isolate up to 25 µg of high-quality plasmid DNA from 1-5 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with HiBind® Mini Column technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.
The E.Z.N.A.® Plasmid DNA Mini Kit is available in two formats – Q-spin (ES52387) and V-spin (ES52388). Q-spin features columns that are capless whereas V-spin includes columns that have a cap attached. The columns are otherwise identical in use and application and can be used in both vacuum or centrifugation protocols.
Features:
- Rapid, purification of plasmid DNA in less than 30 minutes
- Safe, no phenol/chloroform extractions
- Versatile, spin and vacuum formats available
- High-quality, DNA is suitable for a variety of downstream applications
Specifications:
Prep count: 5, 50, 200 preps
Downstream application: Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting material: 1-5 mL LB culture
Plasmid type: High-copy, low-copy, cosmid DNA
Processing mode: Manual (centrifugation or vacuum)
Throughput: 1-24
DNA binding technology: Silica Mini Spin Column
Lysate clearance method: Centrifugation
Processing time: <30 minutes
Yield: 15-25 µg for high copy-number; 0.1-5 µg for low copy-number
Special note: The alternative for discontinued yeast plasmid kit
DNA Concentration Comparison
Endotoxin Level of Purified Plasmid DNA using E.Z.N.A.® Plasmid DNA Mini Kit I
Table 2. Plasmid DNA purified with E.Z.N.A. Plasmid DNA Mini Kit I was used in a 5 µL Sanger sequencing reaction.
Endotoxin Level of Purified Plasmid DNA using E.Z.N.A.® Plasmid DNA Mini Kit I
Table 3. Endotoxins in plasmid DNA preps. Plasmid DNA was isolated from 0.8 mL LB cultures following each manufacturer’s recommended protocols.
Product Documents:
Product Flyer
The E.Z.N.A.® Plasmid DNA Mini Kit is available in two formats – Q-spin (ES52387) and V-spin (ES52388). Q-spin features columns that are capless whereas V-spin includes columns that have a cap attached. The columns are otherwise identical in use and application and can be used in both vacuum or centrifugation protocols.
Features:
- Rapid, purification of plasmid DNA in less than 30 minutes
- Safe, no phenol/chloroform extractions
- Versatile, spin and vacuum formats available
- High-quality, DNA is suitable for a variety of downstream applications
Specifications:
Prep count: 5, 50, 200 preps
Downstream application: Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting material: 1-5 mL LB culture
Plasmid type: High-copy, low-copy, cosmid DNA
Processing mode: Manual (centrifugation or vacuum)
Throughput: 1-24
DNA binding technology: Silica Mini Spin Column
Lysate clearance method: Centrifugation
Processing time: <30 minutes
Yield: 15-25 µg for high copy-number; 0.1-5 µg for low copy-number
Special note: The alternative for discontinued yeast plasmid kit
DNA Concentration Comparison
Figure 1. pGEM plasmid was purified from 4 ml dh5 cultures harboring the plasmid and eluted in 50 l volume using kits from omega bio-tek and company q according to manufacturers recommended protocols.
Super-Coiled DNA
Figure 2. 5 µL of purified pGEM plasmid was analyzed on a 1% Agarose gel. Plasmid was isolated from 1 mL DH5α cultures harboring the plasmid using E.Z.N.A.® Plasmid DNA Mini Kit I.
DNA yield and quality using E.Z.N.A.® Plasmid DNA Mini Kit I
Table 1. pGEM plasmid was purified from 1 mL DH5α cultures harboring the plasmid and eluted in 50 µL volume.
Endotoxin Level of Purified Plasmid DNA using E.Z.N.A.® Plasmid DNA Mini Kit I
Table 2. Plasmid DNA purified with E.Z.N.A. Plasmid DNA Mini Kit I was used in a 5 µL Sanger sequencing reaction.
Endotoxin Level of Purified Plasmid DNA using E.Z.N.A.® Plasmid DNA Mini Kit I
Table 3. Endotoxins in plasmid DNA preps. Plasmid DNA was isolated from 0.8 mL LB cultures following each manufacturer’s recommended protocols.
Product Documents:
Product Flyer
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