Fontana Masson Stain Kit
Details
The Fontana Masson Stain Kit is intended for use in the histological visualization of Argentaffin and Melanin in paraffin or frozen sections.
Argentaffin Cells: Black
Melanin: Black
Nuclei: Red
Cytoplasm: Light Pink
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue:
- Any paraffin embedded tissue that contains Hair Follicles or Skin for Melanin
- Small Intestine for Argentaffin
Preparation of Reagent Prior to Beginning:
Prepare fresh Ammoniacal Silver Solution prior to use.
Using chemically cleaned glassware or new plastic ware, add one vial of Silver Nitrate Solution to 27ml Distilled water and mix. Next add, drop by drop, Concentrated Ammonium Hydroxide (Not included in this kit!); the solution initially turns dark brown and then gradually becomes transparent with a layer of fine sediment. Solution is ready to use when all sediment is dissolved.
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Place freshly mixed Ammoniacal Silver Solution in a 58-60°C waterbath and bring to temperature.
3. Incubate slide in warmed Ammoniacal Silver Solution for 30-60 minutes or until tissue section becomes yellowish/brown in color. (NOTE: Melanin typically stains in 30 minutes while Argentaffin stains in 50-60 minutes)
4. Rinse in several changes of distilled water.
5. Incubate slide in Gold Chloride Solution (0.2%) for 30 seconds.
6. Rinse in several changes of distilled water.
7. Incubate slide in Sodium Thiosulfate Solution (5%) for 1-2 minutes.
8. Rinse for 2 minutes in running tap water followed by 2 changes of distilled water.
9. Incubate slide in Nuclear Fast Red Solution for 5 minutes.
10. Rinse for 2 minutes in running tap water followed by 2 changes of distilled water.
11. Dehydrate very quickly in 3 changes of fresh Absolute Alcohol.
12. Clear, and mount in synthetic resin.
Click for Instructions for Use
Argentaffin Cells: Black
Melanin: Black
Nuclei: Red
Cytoplasm: Light Pink
Uses/Limitations:
- For In-Vitro Diagnostic use only
- Histological applications
- Do not use past expiration date
- Use caution when handling these reagents
Control Tissue:
- Any paraffin embedded tissue that contains Hair Follicles or Skin for Melanin
- Small Intestine for Argentaffin
Preparation of Reagent Prior to Beginning:
Prepare fresh Ammoniacal Silver Solution prior to use.
Using chemically cleaned glassware or new plastic ware, add one vial of Silver Nitrate Solution to 27ml Distilled water and mix. Next add, drop by drop, Concentrated Ammonium Hydroxide (Not included in this kit!); the solution initially turns dark brown and then gradually becomes transparent with a layer of fine sediment. Solution is ready to use when all sediment is dissolved.
Procedure (Standard):
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Place freshly mixed Ammoniacal Silver Solution in a 58-60°C waterbath and bring to temperature.
3. Incubate slide in warmed Ammoniacal Silver Solution for 30-60 minutes or until tissue section becomes yellowish/brown in color. (NOTE: Melanin typically stains in 30 minutes while Argentaffin stains in 50-60 minutes)
4. Rinse in several changes of distilled water.
5. Incubate slide in Gold Chloride Solution (0.2%) for 30 seconds.
6. Rinse in several changes of distilled water.
7. Incubate slide in Sodium Thiosulfate Solution (5%) for 1-2 minutes.
8. Rinse for 2 minutes in running tap water followed by 2 changes of distilled water.
9. Incubate slide in Nuclear Fast Red Solution for 5 minutes.
10. Rinse for 2 minutes in running tap water followed by 2 changes of distilled water.
11. Dehydrate very quickly in 3 changes of fresh Absolute Alcohol.
12. Clear, and mount in synthetic resin.
Click for Instructions for Use
| Components | Size | SDS Links |
|---|---|---|
| Gold Chloride Solution (0.2%) | 125 mL | View SDS |
| Silver Nitrate Solution (10%) | 5 x 9 mL | View SDS |
| Sodium Thiosulfate Solution (5%) | 125 mL | View SDS |
| Nuclear Fast Red Solution | 125 mL | View SDS |
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